Association of 2238T>C Polymorphism of the Atrial Natriuretic Peptide Gene With Coronary Artery Disease in Afro-Caribbeans With Type 2 Diabetes

• Published in: American journal of hypertension 2012-05, Vol.25 (5), p.524-527
• Main Authors: Larifla, Laurent, Maimaitiming, Suriya, Velayoudom-Cephise, Fritz-Line, Ferdinand, Severine, Blanchet-Deverly, Anne, BenAbdallah, Sonia, Donnet, Jean-Paul, Atallah, André, Roussel, Ronan, Foucan, Lydia
• Format: Article
• Language: English
• Subjects: African Continental Ancestry Group - ethnology; African Continental Ancestry Group - genetics; Aged; Arterial hypertension. Arterial hypotension; Atrial Natriuretic Factor - genetics; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cardiovascular Diseases - epidemiology; Clinical manifestations. Epidemiology. Investigative techniques. Etiology; Comorbidity; Coronary Artery Disease - epidemiology; Coronary Artery Disease - ethnology; Coronary Artery Disease - genetics; Cross-Sectional Studies; Diabetes Mellitus, Type 2 - epidemiology; Diabetes Mellitus, Type 2 - ethnology; Diabetes Mellitus, Type 2 - genetics; Female; Genotype; Humans; Male; Medical sciences; Middle Aged; Polymorphism, Single Nucleotide - genetics; Regression Analysis; Retrospective Studies; Risk Factors; West Indies
• ISSN: 0895-7061; 1941-7225; 1879-1905
• DOI: 10.1038/ajh.2011.233

Background The atrial natriuretic peptide (ANP) is known mainly for its effects on kidney function and blood pressure homeostasis. We investigated the association between two ANP polymorphisms and pre-existing coronary artery disease (CAD) in patients of African descent with type 2 diabetes (T2D). Methods We conducted a cross-sectional and retrospective study of 218 volunteer Afro-Caribbean patients with T2D. Two polymorphisms (rs5064, 708C>T; and rs5065, 2238T>C) of ANP were genotyped using PCR-restriction fragment length polymorphism analysis. ANCOVA, χ2-test, and logistic regression were used for statistical analysis. Results Among these patients (92 men; 128 women), 67 (30.7%) had CAD, of whom 75% had had myocardial infarction. The frequency of rs5065-C carriers (TC/CC) was significantly lower in patients with CAD than in those without CAD (24 vs. 41%, P = 0.01). The frequency of hypertension did not differ significantly according to genotype. Univariate logistic regression revealed that male sex, age, dyslipidemia, hypertension, and rs5065-C carrier status were associated significantly with CAD. After adjustment for the variables of interest, the odds ratio (ORs) of CAD for rs5065-C carriers (TC/CC) was 0.50 (0.26-0.96; P = 0.038). No association was found between the rs5064 (708C>T) single-nucleotide polymorphisms (SNPs) and pre-existing CAD or cardiovascular risk factors. Conclusions The ANP rs5065 (2238T>C) C allele seems to exert a protective effect against CAD in T2D patients of African descent. The relevance of ANP polymorphisms for CAD should be determined in different populations.