Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease

A major function of the enzyme intestinal alkaline phosphatase (iAP) is the detoxification of lipopolysaccharide (LPS), the ligand of Toll-like receptor 4 (TLR4). Hence, iAP has a role in the defence of maintaining intestinal barrier integrity. As intestinal barrier integrity is impaired in coeliac...

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Bibliographic Details
Published in:Virchows Archiv : an international journal of pathology 2012-02, Vol.460 (2), p.157-161
Main Authors: Molnár, Kriszta, Vannay, Ádám, Sziksz, Erna, Bánki, Nóra Fanni, Győrffy, Hajnalka, Arató, András, Dezsőfi, Antal, Veres, Gabor
Format: Article
Language:English
Subjects:
Adolescent
Alkaline Phosphatase - biosynthesis
Biological and medical sciences
Blotting, Western
Celiac Disease - enzymology
Child
Child, Preschool
Detoxification
Female
Fluorescent Antibody Technique
Gastroenterology. Liver. Pancreas. Abdomen
Gluten
Humans
Infant
Intestinal Mucosa - enzymology
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Medicine
Medicine & Public Health
Original Article
Other diseases. Semiology
Pathology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Real-Time Polymerase Chain Reaction
RNA, Messenger - analysis
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Toll-Like Receptor 4 - biosynthesis
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Summary:A major function of the enzyme intestinal alkaline phosphatase (iAP) is the detoxification of lipopolysaccharide (LPS), the ligand of Toll-like receptor 4 (TLR4). Hence, iAP has a role in the defence of maintaining intestinal barrier integrity. As intestinal barrier integrity is impaired in coeliac disease (CD), we tested the expression and localization of iAP in duodenal mucosa specimens from children with newly diagnosed CD ( n  = 10), with CD on gluten-free diet (GFD) ( n  = 5) and compared to those from ten healthy children. The mRNA and protein expression was determined by RT-PCR and Western blot analysis, respectively. Tissue localization of iAP and TLR4 was determined by immunofluorescence staining. iAP protein expression level was significantly lower than normal in newly diagnosed CD, while it was normalised in children on GFD. iAP and TLR4 colocalized at the epithelial surface of duodenal mucosa in each group of subjects enrolled. The finding of decreased iAP protein levels in newly diagnosed CD is consistent with its role in decreased intestinal barrier integrity. The latter may be the result of decreased LPS-detoxifying ability.
ISSN:0945-6317
1432-2307
DOI:10.1007/s00428-011-1188-5