Significance analysis of xMap cytokine bead arrays

Highly multiplexed assays using antibody coated, fluorescent (xMap) beads are widely used to measure quantities of soluble analytes, such as cytokines and antibodies in clinical and other studies. Current analyses of these assays use methods based on standard curves that have limitations in detectin...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2012-02, Vol.109 (8), p.2848-2853
Main Authors: Won, Joong-Ho, Goldberger, Ofir, Shen-Orr, Shai S, Davis, Mark M, Olshen, Richard A
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Cytokines
Cytokines - analysis
Cytokines - blood
False positive errors
Fluorescence
Francisella tularensis
Francisella tularensis - physiology
gene expression regulation
Humans
Infections
Juvenile rheumatoid arthritis
Mice
Microspheres
Models, Biological
Models, Statistical
Physical Sciences
Protein Array Analysis - methods
Proteins
Research universities
Statistical discrepancies
Systemic lupus erythematosus
Tularemia - blood
Tularemia - immunology
Universities
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Summary:Highly multiplexed assays using antibody coated, fluorescent (xMap) beads are widely used to measure quantities of soluble analytes, such as cytokines and antibodies in clinical and other studies. Current analyses of these assays use methods based on standard curves that have limitations in detecting low or high abundance analytes. Here we describe SAxCyB (Significance Analysis of xMap Cytokine Beads), a method that uses fluorescence measurements of individual beads to find significant differences between experimental conditions. We show that SAxCyB outperforms conventional analysis schemes in both sensitivity (low fluorescence) and robustness (high variability) and has enabled us to find many new differentially expressed cytokines in published studies.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1112599109