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Identification of genes affecting apolipoprotein B secretion following siRNA-mediated gene knockdown in primary human hepatocytes
Highlights ► siRNA-mediated gene knockdown in primary human hepatocytes was used to identify potential causal genes affecting lipid traits. ► Approximately 100 GWAS loci significantly affecting human plasma lipid levels were identified. ► A total of 191 genes from 74 loci were selected and subjected...
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| Published in: | Atherosclerosis 2012-05, Vol.222 (1), p.154-157 |
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| Main Authors: | , , , , , , , , , |
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| container_end_page | 157 |
| container_issue | 1 |
| container_start_page | 154 |
| container_title | Atherosclerosis |
| container_volume | 222 |
| creator | Shen, Xun Wang, Wei Wang, Liangsu Houde, Caroline Wu, Weizhen Tudor, Matt Thompson, John R Sisk, Christine McCrary Hubbard, Brian Li, Jing |
| description | Highlights ► siRNA-mediated gene knockdown in primary human hepatocytes was used to identify potential causal genes affecting lipid traits. ► Approximately 100 GWAS loci significantly affecting human plasma lipid levels were identified. ► A total of 191 genes from 74 loci were selected and subjected to siRNA-mediated gene knockdown. ► Four genes (PARP10, HP, FUT1, and LPAR2) were identified and verified as affecting ApoB secretion. |
| doi_str_mv | 10.1016/j.atherosclerosis.2012.02.012 |
| format | article |
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Vascular system ; Cardiovascular ; Cholesterol ; Cholesterol, HDL - blood ; Cholesterol, LDL - blood ; Fucosyltransferases - genetics ; Galactoside 2-alpha-L-fucosyltransferase ; Gene Knockdown Techniques ; genes ; Genome-Wide Association Study ; Haptoglobins - genetics ; hepatocytes ; Hepatocytes - metabolism ; high density lipoprotein cholesterol ; Humans ; Lipids ; loci ; low density lipoprotein cholesterol ; Medical sciences ; Poly(ADP-ribose) Polymerases - genetics ; Proprotein Convertase 9 ; Proprotein Convertases - metabolism ; Proto-Oncogene Proteins - genetics ; Receptors, Lysophosphatidic Acid - genetics ; RNA, Small Interfering - pharmacology ; secretion ; Serine Endopeptidases - metabolism ; siRNA ; small interfering RNA ; subtilisin ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the heart ; triacylglycerols ; Triglycerides - blood</subject><ispartof>Atherosclerosis, 2012-05, Vol.222 (1), p.154-157</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2012 Elsevier Ireland Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-827b848edea444795761fc725926f91472e74773961f39a2b6cab5dec91b3cfa3</citedby><cites>FETCH-LOGICAL-c498t-827b848edea444795761fc725926f91472e74773961f39a2b6cab5dec91b3cfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25820382$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22398276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Xun</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Wang, Liangsu</creatorcontrib><creatorcontrib>Houde, Caroline</creatorcontrib><creatorcontrib>Wu, Weizhen</creatorcontrib><creatorcontrib>Tudor, Matt</creatorcontrib><creatorcontrib>Thompson, John R</creatorcontrib><creatorcontrib>Sisk, Christine McCrary</creatorcontrib><creatorcontrib>Hubbard, Brian</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><title>Identification of genes affecting apolipoprotein B secretion following siRNA-mediated gene knockdown in primary human hepatocytes</title><title>Atherosclerosis</title><addtitle>Atherosclerosis</addtitle><description>Highlights ► siRNA-mediated gene knockdown in primary human hepatocytes was used to identify potential causal genes affecting lipid traits. ► Approximately 100 GWAS loci significantly affecting human plasma lipid levels were identified. ► A total of 191 genes from 74 loci were selected and subjected to siRNA-mediated gene knockdown. ► Four genes (PARP10, HP, FUT1, and LPAR2) were identified and verified as affecting ApoB secretion.</description><subject>Apolipoprotein A-I - metabolism</subject><subject>Apolipoprotein B</subject><subject>Apolipoproteins B - metabolism</subject><subject>Atherosclerosis</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>blood lipids</subject><subject>Cardiology. Vascular system</subject><subject>Cardiovascular</subject><subject>Cholesterol</subject><subject>Cholesterol, HDL - blood</subject><subject>Cholesterol, LDL - blood</subject><subject>Fucosyltransferases - genetics</subject><subject>Galactoside 2-alpha-L-fucosyltransferase</subject><subject>Gene Knockdown Techniques</subject><subject>genes</subject><subject>Genome-Wide Association Study</subject><subject>Haptoglobins - genetics</subject><subject>hepatocytes</subject><subject>Hepatocytes - metabolism</subject><subject>high density lipoprotein cholesterol</subject><subject>Humans</subject><subject>Lipids</subject><subject>loci</subject><subject>low density lipoprotein cholesterol</subject><subject>Medical sciences</subject><subject>Poly(ADP-ribose) Polymerases - genetics</subject><subject>Proprotein Convertase 9</subject><subject>Proprotein Convertases - metabolism</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Receptors, Lysophosphatidic Acid - genetics</subject><subject>RNA, Small Interfering - pharmacology</subject><subject>secretion</subject><subject>Serine Endopeptidases - metabolism</subject><subject>siRNA</subject><subject>small interfering RNA</subject><subject>subtilisin</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the heart</subject><subject>triacylglycerols</subject><subject>Triglycerides - blood</subject><issn>0021-9150</issn><issn>1879-1484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkk1v1DAQhiMEokvhL0AulbhksZ0v-wBSqaBUqkCi9Gw5znjXu147tROqPfLPmXSXHnpC8odkPfPOeN7JsjNKlpTQ5sNmqcY1xJC0m0-bloxQtiS4KHuWLShvRUErXj3PFoQwWghak5PsVUobQkjVUv4yO2GsFJy1zSL7c9WDH62xWo02-DyYfAUeUq6MAT1av8rVEJwdwhDDCNbnn_MEOsIDbYJz4X6Gkv35_bzYQW_VCP2DRr71QW_7cO9zDBui3am4z9fTTvl8DYMag96PkF5nL4xyCd4c79Ps9uuXXxffiusfl1cX59eFrgQfCyy34xWHHlRVVa2o24Ya3bJasMYIWrUM2qptS4HPpVCsa7Tq6h60oF2pjSpPs_cHXfzI3QRplDubNDinPIQpSUqIoIzzpkT04wHV2OEUwchj9QjJ2QW5kU9ckLMLkuCiDOPfHlNNHbbkMfpf2xE4OwIqaeVMVF6jxiNXc0ZKPgu9O3BGBalWEZnbG8xUz1byWnAkLg8EYOt-W4gyaQteoxER_ZN9sP9d9KcnStpZj3PhtrCHtAlT9OiPpDJhgLyZh2ueLcoI7rop_wKap9Ah</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Shen, Xun</creator><creator>Wang, Wei</creator><creator>Wang, Liangsu</creator><creator>Houde, Caroline</creator><creator>Wu, Weizhen</creator><creator>Tudor, Matt</creator><creator>Thompson, John R</creator><creator>Sisk, Christine McCrary</creator><creator>Hubbard, Brian</creator><creator>Li, Jing</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>Identification of genes affecting apolipoprotein B secretion following siRNA-mediated gene knockdown in primary human hepatocytes</title><author>Shen, Xun ; Wang, Wei ; Wang, Liangsu ; Houde, Caroline ; Wu, Weizhen ; Tudor, Matt ; Thompson, John R ; Sisk, Christine McCrary ; Hubbard, Brian ; Li, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-827b848edea444795761fc725926f91472e74773961f39a2b6cab5dec91b3cfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Apolipoprotein A-I - metabolism</topic><topic>Apolipoprotein B</topic><topic>Apolipoproteins B - metabolism</topic><topic>Atherosclerosis</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>blood lipids</topic><topic>Cardiology. Vascular system</topic><topic>Cardiovascular</topic><topic>Cholesterol</topic><topic>Cholesterol, HDL - blood</topic><topic>Cholesterol, LDL - blood</topic><topic>Fucosyltransferases - genetics</topic><topic>Galactoside 2-alpha-L-fucosyltransferase</topic><topic>Gene Knockdown Techniques</topic><topic>genes</topic><topic>Genome-Wide Association Study</topic><topic>Haptoglobins - genetics</topic><topic>hepatocytes</topic><topic>Hepatocytes - metabolism</topic><topic>high density lipoprotein cholesterol</topic><topic>Humans</topic><topic>Lipids</topic><topic>loci</topic><topic>low density lipoprotein cholesterol</topic><topic>Medical sciences</topic><topic>Poly(ADP-ribose) Polymerases - genetics</topic><topic>Proprotein Convertase 9</topic><topic>Proprotein Convertases - metabolism</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Receptors, Lysophosphatidic Acid - genetics</topic><topic>RNA, Small Interfering - pharmacology</topic><topic>secretion</topic><topic>Serine Endopeptidases - metabolism</topic><topic>siRNA</topic><topic>small interfering RNA</topic><topic>subtilisin</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. 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| ispartof | Atherosclerosis, 2012-05, Vol.222 (1), p.154-157 |
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| subjects | Apolipoprotein A-I - metabolism Apolipoprotein B Apolipoproteins B - metabolism Atherosclerosis Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels blood lipids Cardiology. Vascular system Cardiovascular Cholesterol Cholesterol, HDL - blood Cholesterol, LDL - blood Fucosyltransferases - genetics Galactoside 2-alpha-L-fucosyltransferase Gene Knockdown Techniques genes Genome-Wide Association Study Haptoglobins - genetics hepatocytes Hepatocytes - metabolism high density lipoprotein cholesterol Humans Lipids loci low density lipoprotein cholesterol Medical sciences Poly(ADP-ribose) Polymerases - genetics Proprotein Convertase 9 Proprotein Convertases - metabolism Proto-Oncogene Proteins - genetics Receptors, Lysophosphatidic Acid - genetics RNA, Small Interfering - pharmacology secretion Serine Endopeptidases - metabolism siRNA small interfering RNA subtilisin Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the heart triacylglycerols Triglycerides - blood |
| title | Identification of genes affecting apolipoprotein B secretion following siRNA-mediated gene knockdown in primary human hepatocytes |
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