Mass spectrometry based methods for the discrimination and typing of mycobacteria

Identification and typing of mycobacteria is very important for epidemiology, susceptibility testing and diagnostic purposes. This paper describes the development and validation of the alternative methods for species identification and typing of mycobacteria based on a matrix-assisted laser desorpti...

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Bibliographic Details
Published in:Infection, genetics and evolution genetics and evolution, 2012-06, Vol.12 (4), p.838-845
Main Authors: Shitikov, E., Ilina, E., Chernousova, L., Borovskaya, A., Rukin, I., Afanas’ev, M., Smirnova, T., Vorobyeva, A., Larionova, E., Andreevskaya, S., Kostrzewa, M., Govorun, V.
Format: Article
Language:English
Subjects:
Animals
Bacterial Typing Techniques
Base Sequence
cluster analysis
Direct bacterial profiling
epidemiology
Humans
ionization
MALDI
mass spectrometry
Molecular Sequence Data
Mycobacterium
Mycobacterium - classification
Mycobacterium tuberculosis
Nontuberculous mycobacteria
species identification
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spoligotyping
Tuberculosis
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Summary:Identification and typing of mycobacteria is very important for epidemiology, susceptibility testing and diagnostic purposes. This paper describes the development and validation of the alternative methods for species identification and typing of mycobacteria based on a matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-ToF MS). Altogether there were 383 clinical isolates analyzed which include 348 strains of Mycobacterium tuberculosis complex (MTBC) (342 strains of M. tuberculosis and 6 strains of M. bovis) and 35 strains of nontuberculous mycobacteria (NTM) represented by 16 different species. Direct bacterial profiling (DBP) by means of MALDI-ToF MS was carried out. Cluster analysis of DBP mass spectra divided them into two large separate groups corresponding to MTBC and NTM, and also demonstrated the possibility of isolate identification at the species level. Spoligotyping protocol based on mass spectrometry was developed and validated, it matched completely to classical spoligotyping data. Our results suggest that MALDI-ToF MS has potential as a rapid and reproducible platform for the identification and typing of Mycobacterium species.
ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2011.12.013