DNA barcoding of Oryx leucoryx using the mitochondrial cytochrome C oxidase gene

The massive destruction and deterioration of the habitat of Oryx leucoryx and illegal hunting have decimated Oryx populations significantly, and now these animals are almost extinct in the wild. Molecular analyses can significantly contribute to captive breeding and reintroduction strategies for the...

Full description

Saved in:
Bibliographic Details
Published in:Genetics and molecular research 2012-01, Vol.11 (1), p.539-547
Main Authors: Elmeer, K, Almalki, A, Mohran, K A, Al-Qahtani, K N, Almarri, M
Format: Article
Language:English
Subjects:
Animals
Antelopes - classification
Antelopes - genetics
Base Sequence
DNA Barcoding, Taxonomic
DNA, Mitochondrial
Electron Transport Complex IV - genetics
Female
Male
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S - chemistry
Sequence Analysis, DNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The massive destruction and deterioration of the habitat of Oryx leucoryx and illegal hunting have decimated Oryx populations significantly, and now these animals are almost extinct in the wild. Molecular analyses can significantly contribute to captive breeding and reintroduction strategies for the conservation of this endangered animal. A representative 32 identical sequences used for species identification through BOLD and GenBank/NCBI showed maximum homology 96.06% with O. dammah, which is a species of Oryx from Northern Africa, the next closest species 94.33% was O. gazella, the African antelope. DNA barcode sequences of the mitochondrial cytochrome C oxidase (COI) gene were determined for O. leucoryx; identification through BOLD could only recognize the genus correctly, whereas the species could not be identified. This was due to a lack of sequence data for O. leucoryx on BOLD. Similarly, BLAST analysis of the NCBI data base also revealed no COI sequence data for the genus Oryx.
ISSN:1676-5680
1676-5680
DOI:10.4238/2012.March.8.2