Immunohistochemical localization of somatostatin and pancreatic polypeptide in smokers with chronic pancreatitis

Recent studies have demonstrated a significant role of tobacco smoking in the development of chronic pancreatitis. Although there are published papers on the effects of cigarette smoking on insulin secretion in patients, no data are available on the effects of smoking on pancreatic endocrine cells s...

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Bibliographic Details
Published in:Acta histochemica 2012-09, Vol.114 (5), p.495-502
Main Authors: Sliwinska-Mosson, Mariola, Milnerowicz, Halina, Milnerowicz, Stanislaw, Nowak, Marcin, Rabczynski, Jerzy
Format: Article
Language:English
Subjects:
Adult
Chronic pancreatitis
Female
Humans
Immunohistochemistry
Islets of Langerhans - chemistry
Islets of Langerhans - pathology
Male
Middle Aged
Pancreatic polypeptide
Pancreatic Polypeptide - analysis
Pancreatitis, Chronic - complications
Pancreatitis, Chronic - metabolism
Pancreatitis, Chronic - pathology
Smoking
Smoking - metabolism
Somatostatin
Somatostatin - analysis
Young Adult
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Summary:Recent studies have demonstrated a significant role of tobacco smoking in the development of chronic pancreatitis. Although there are published papers on the effects of cigarette smoking on insulin secretion in patients, no data are available on the effects of smoking on pancreatic endocrine cells secreting somatostatin and pancreatic polypeptide. The aim of the study was to evaluate the effects of cigarette smoking on endocrine pancreatic function by immunolocalization of somatostatin and pancreatic polypeptide in the pancreas from smokers and non-smoking patients with chronic pancreatitis in comparison with healthy controls. The LSAB2-HRP technique with polyclonal antibodies was used for the immunolocalization of somatostatin and pancreatic polypeptide in histological preparations of the pancreas. The intensity of immunohistochemical reaction was calculated with digital image analysis. The study demonstrated increased numbers of somatostatin (D) secreting cells and pancreatic polypeptide (PP) cells and their altered location in pancreatic islets and parenchyma of smoking patients with chronic pancreatitis, as compared to non-smoking patients and healthy controls. Smoking patients showed significantly higher immunostaining of the hormones in the pancreas compared to non-smoking patients and healthy persons. This study indicates that smoking may play a significant role in the development of endocrine disturbances in the development of chronic pancreatitis.
ISSN:0065-1281
1618-0372
DOI:10.1016/j.acthis.2011.09.003