Minimal increase in genetic diversity enhances predation resistance

• Published in: Molecular ecology 2012-04, Vol.21 (7), p.1741-1753
• Main Authors: KOH, KAI S., MATZ, CARSTEN, TAN, CHUAN H., LE, HOANG L., RICE, SCOTT A., MARSHALL, DUSTIN J., STEINBERG, PETER D., KJELLEBERG, STAFFAN
• Format: Article
• Language: English
• Subjects: Bacteria; bacterial biofilms; Biofilms; community resilience; diversity; DNA, Bacterial - genetics; ecological complementarity; Gene Knockout Techniques; Genetic diversity; Genetic Variation; Genetics, Population; Genotype; Genotype & phenotype; Phenotype; Polymorphism, Single Nucleotide; Predation; protozoan grazing; Serratia marcescens; Serratia marcescens - genetics; Serratia marcescens - physiology; Tetrahymena pyriformis - physiology
• ISSN: 0962-1083; 1365-294X
• DOI: 10.1111/j.1365-294X.2011.05415.x

The importance of species diversity to emergent, ecological properties of communities is increasingly appreciated, but the importance of within‐species genetic diversity for analogous emergent properties of populations is only just becoming apparent. Here, the properties and effects of genetic variation on predation resistance in populations were assessed and the molecular mechanism underlying these emergent effects was investigated. Using biofilms of the ubiquitous bacterium Serratia marcescens, we tested the importance of genetic diversity in defending biofilms against protozoan grazing, a main source of mortality for bacteria in all natural ecosystems. S. marcescens biofilms established from wild‐type cells produce heritable, stable variants, which when experimentally combined, persist as a diverse assemblage and are significantly more resistant to grazing than either wild type or variant biofilms grown in monoculture. This diversity effect is biofilm‐specific, a result of either facilitation or resource partitioning among variants, with equivalent experiments using planktonic cultures and grazers resulting in dominance by a single resistant strain. The variants studied are all the result of single nucleotide polymorphisms in one regulatory gene suggesting that the benefits of genetic diversity in clonal biofilms can occur through remarkably minimal genetic change. The findings presented here provide a new insight on the integration of genetics and population ecology, in which diversity arising through minimal changes in genotype can have major ecological implications for natural populations. See also the Perspective by Crutsinger