A novel bridge between oxidative stress and immunity: the interaction between hydrogen peroxide and human leukocyte antigen G in placental trophoblasts during preeclampsia

Objective The aim of this study was to investigate a possible association between hydrogen peroxide and human leukocyte antigen G (HLA-G) in preeclampsia. Study Design We explored the correlation between HLA-G and hydrogen peroxide in preeclamptic (n = 30) and normotensive (n = 30) placentas, which...

Full description

Saved in:
Bibliographic Details
Published in:American journal of obstetrics and gynecology 2012-05, Vol.206 (5), p.447.e7-447.e16
Main Authors: Zhou, Xue, MD, PhD, Zhang, Guo-ying, MD, Wang, Jue, MD, Lu, Shou-lian, MD, Cao, Jun, Sun, Li-zhou, MD, PhD
Format: Article
Language:English
Subjects:
Adult
Apoptosis
Biomarkers - metabolism
Blotting, Western
Case-Control Studies
Cell Line
Cell Survival
Down-Regulation
Female
Fluorescent Antibody Technique
HLA-G Antigens - metabolism
human leukocyte antigen-G
Humans
hydrogen peroxide
Hydrogen Peroxide - metabolism
Obstetrics and Gynecology
Oxidative Stress
Placenta - metabolism
Pre-Eclampsia - etiology
Pre-Eclampsia - immunology
preeclampsia
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
siRNA
trophoblast
Trophoblasts - metabolism
Trophoblasts - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective The aim of this study was to investigate a possible association between hydrogen peroxide and human leukocyte antigen G (HLA-G) in preeclampsia. Study Design We explored the correlation between HLA-G and hydrogen peroxide in preeclamptic (n = 30) and normotensive (n = 30) placentas, which was confirmed by in vitro experiments. Furthermore, RNA interference was used to explore the influence of HLA-G on the proliferation, apoptosis and invasion of HTR-8/SVneo cells when exposed to hydrogen peroxide. Results We found an inverse correlation between hydrogen peroxide and HLA-G expression in preeclamptic placentas. High levels of hydrogen peroxide could down-regulate HLA-G expression in HTR-8/SVneo. Compared with HLA-G knockdown HTR-8/SVneo, increased proliferation inhibition, higher apoptosis, and decreased cell invasion were found in the cell expressing HLA-G when exposed to hydrogen peroxide. Conclusion Our findings highlight that high levels of hydrogen peroxide can down-regulate HLA-G expression in trophoblasts during preeclampsia and trophoblasts expressing HLA-G are vulnerable to oxidative stress.
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2012.03.013