Imipramine Inhibits Adipogenic Differentiation in Both 3T3-L1 Preadipocytes and Mouse Marrow Stromal Cells

• Published in: Journal of genetics and genomics 2012-04, Vol.39 (4), p.173-180
• Main Authors: Li, Haifang, Fong, Chi-chun, Chen, Yao, Cai, Guoping, Yang, Mengsu
• Format: Article
• Language: English
• Subjects: 3T3 Cells; 3T3-L1 preadipocytes; Adipocytes - cytology; Adipocytes - metabolism; Adipogenesis; Adipogenic differentiation; Animals; Biomarkers - metabolism; Blotting, Western; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Cell Survival; Female; Gene Expression Regulation; Imipramine; Imipramine - pharmacology; Marrow stromal cells (MSCs); Mice; mRNA表达; PPAR gamma - genetics; PPAR gamma - metabolism; Receptors, Adrenergic, beta - genetics; Receptors, Adrenergic, beta - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stromal Cells - cytology; Stromal Cells - drug effects; Stromal Cells - metabolism; 分化; 前脂肪细胞; 小鼠; 过氧化物酶体增殖物激活受体; 骨髓基质干细胞; 骨髓基质细胞; 骨髓间充质干细胞
• ISSN: 1673-8527
• DOI: 10.1016/j.jgg.2012.03.003

Imipramine （IM） has been widely used clinically for the treatment of mental disorders. Its actions on tissues or organs other than the nervous system also need to be understood for its proper clinical use. In this study, the effects of IM on adipogenic differentiation in both 3T3-L1 preadipocytes and mouse marrow stromal cells （MSCs） were investigated. The results showed that fewer adipocytic cells were developed from 3T3-L1 preadipocytes in the presence of 0.001 to 1 μmol/L of IM as compared to control. Similar inhibitory effect was also observed in mouse MSCs. The decrease in the formation of adipocytes was accompanied with significant down-regulation at mRNA expression of the early adipogenic transcription factor, peroxisome proliferator-activated receptor γ2 （PPARγ2）. Western blot analysis further revealed that the protein expression of PPARγ2 was reduced markedly in ceils treated with IM at concentrations of 0.01, 0.1 and 1 μmol/L, suggesting that the suppression on PPAR72 was involved in IM＇s inhibition on MSCs adipogenesis. Moreover, IM at the above concentrations could stimulate the mRNA expression of β2-adrenergic receptor （AR） and β3-AR, which implicated that the effect of IM on adipogenic differentiation was partially mediated by β-ARs. Our results demonstrated for the first time that the conventional antidepressive imipramine exerts accompanied inhibitory effect on adipocyte formation, which may have possible clinical implications.