Effects of Amifostine in Combination With Cyclophosphamide on Female Reproductive System

This study investigated the cytoprotective effects of amifostine against the adverse effects of cyclophosphamide relying on ovarian cell death markers and the fertilization rate of the surviving follicles as a late outcome of the study. Combined pretreatment of amifostine with cyclophosphamide enabl...

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Bibliographic Details
Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2012-05, Vol.19 (5), p.539-546
Main Authors: Barekati, Zeinab, Golkar-Narenji, Afsaneh, Totonchi, Mehdi, Radpour, Ramin, Gourabi, Hamid
Format: Article
Language:English
Subjects:
Amifostine - administration & dosage
Animals
Antineoplastic Agents, Alkylating - adverse effects
Apoptosis - drug effects
bcl-2-Associated X Protein - genetics
Biological and medical sciences
Caspase 3 - genetics
Cyclophosphamide - administration & dosage
Cyclophosphamide - adverse effects
Cytoprotection - drug effects
Embryo Culture Techniques
Embryology
Female
Fertility Preservation - methods
Fertilization in Vitro
Gynecology. Andrology. Obstetrics
In Situ Nick-End Labeling
Medical sciences
Medicine & Public Health
Mice
Mice, Inbred BALB C
Obstetrics/Perinatology/Midwifery
Oocytes - cytology
Oocytes - drug effects
Oocytes - physiology
Original Articles
Ovarian Follicle - drug effects
Ovary - drug effects
Reproductive Medicine
Reverse Transcriptase Polymerase Chain Reaction
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Summary:This study investigated the cytoprotective effects of amifostine against the adverse effects of cyclophosphamide relying on ovarian cell death markers and the fertilization rate of the surviving follicles as a late outcome of the study. Combined pretreatment of amifostine with cyclophosphamide enabled partial recovery of antiapoptotic messenger RNA (mRNA) expression levels while a decrease in expression of BAX and Casp3 were identified. The pretreatment of amifostine to cyclophosphamide significantly reduced the proportion of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive primary and preantral follicles (P < .001 and P < .05, respectively). These findings were comparable with the results obtained from protein expression of cleaved caspase 3. The fertilization rate showed a significant capability of amifostine to improve fertilization potency of oocytes exposed to cyclophosphamide (P < .01). In conclusion, administration of amifostine prior to cyclophosphamide might serve as a promising protocol to improve female fertility.
ISSN:1933-7191
1933-7205
DOI:10.1177/1933719111426599