Isolation and characterization of 10 SSR markers of Momordica charantia (Cucurbitaceae)

• Premise of the study: Microsatellite markers were isolated and characterized from the genome of Momordica charantia (bitter melon) to be applied in studies of genetic diversity and population structure. • Methods and Results: Twenty‐five microsatellite loci were isolated from the genome of bitter...

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Bibliographic Details
Published in:American journal of botany 2012-05, Vol.99 (5), p.e182-e183
Main Authors: Guo, Da‐Long, Zhang, Ju‐Ping, Xue, Yi‐Min, Hou, Xiao‐Gai
Format: Article
Language:English
Subjects:
Comparative analysis
Cucurbita pepo
Cucurbitaceae
DNA Primers - metabolism
DNA, Plant - genetics
DNA, Plant - isolation & purification
FIASCO
Genetic diversity
Genomics
microsatellite
Microsatellite Repeats - genetics
Molecular Sequence Data
Momordica charantia
Momordica charantia - genetics
Momordica cochinchinensis
Plant populations
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Summary:• Premise of the study: Microsatellite markers were isolated and characterized from the genome of Momordica charantia (bitter melon) to be applied in studies of genetic diversity and population structure. • Methods and Results: Twenty‐five microsatellite loci were isolated from the genome of bitter melon using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) method. Ten loci were polymorphic, and the number of alleles per locus ranged from three to seven, with the observed heterozygosity ranging from 0.46 to 0.65. The markers also amplified successfully in the related species M. cochinchinensis and Cucurbita pepo. • Conclusions: These markers will have potential utility for applications in genetic diversity evaluation, molecular fingerprinting, identification, comparative genomics analysis, and genetic mapping in Momordica species, as well as in C. pepo.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1100277