Increase of choroidal and scleral MCP-1 expression in hypercholesterolemia experimental model

The aim of this study is to experientially demonstrate that a cholesterol-enriched diet induces an increase in the MCP-1 expression in the choroid and sclera. New Zealand rabbits were divided into two groups: GN (normal diet group) of 8 rabbits (8 eyes) was fed a standard rabbit diet for 4 weeks; GH...

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Bibliographic Details
Published in:Arquivos brasileiros de oftalmologia 2012-01, Vol.75 (1), p.48-52
Main Authors: Torres, Rogil José de Almeida, Noronha, Lucia de, Casella, Antonio Marcelo Barbante, Lumikoski, Thaís Isabel, Précoma, Leonardo Brandão, Torres, Caroline Luzia de Almeida, Luchini, Andréa, Sturzeneker, Mario Claudio Soares, Précoma, Dalton Bertolim
Format: Article
Language:Portuguese
Subjects:
Animals
Chemokine CCL2 - metabolism
Cholesterol, Dietary - adverse effects
Choroid - metabolism
Disease Models, Animal
Hypercholesterolemia - etiology
Hypercholesterolemia - metabolism
Immunohistochemistry
Male
Rabbits
Sclera - metabolism
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Summary:The aim of this study is to experientially demonstrate that a cholesterol-enriched diet induces an increase in the MCP-1 expression in the choroid and sclera. New Zealand rabbits were divided into two groups: GN (normal diet group) of 8 rabbits (8 eyes) was fed a standard rabbit diet for 4 weeks; GH (hypercholesterolemic group) of 13 rabbits (13 eyes) was fed a 1% cholesterol enriched diet for 8 weeks. Total serum cholesterol, triglyceride, HDL cholesterol and fasting blood glucose exams were performed at the start of the experiment and at the euthanasia time. After GH 8th week and GN 4th week animals were euthanized and their eyes underwent immunohistochemical analysis with the anti-MCP-1 antibody. The diet has induced a significant increase in GH total cholesterol and triglyceride levels when compared with NG (p
ISSN:1678-2925