The Greenbug, Schizaphis graminum Rondani (Hemiptera: Aphididae), in Tunisia: Mitochondrial DNA Divergence and Haplotype Inference

The greenbug, Schizaphis graminum (Rondani), is a major pest of wheat worldwide. Based on host plant response to infestation, several biotypes of this pest have been identified and their phylogenetic relationships inferred using the mitochondrial COI gene. In this study, we have sequenced a fragment...

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Bibliographic Details
Published in:Journal of Entomological Research Society 2012-01, Vol.14 (1), p.77-82
Main Authors: Kharrat, Imen, Mezghani-Khemakhem, Maha, Bouktila, Dhia, Makni, Hanem, Makni, Mohamed
Format: Article
Language:English
Subjects:
Aphididae
Biotypes
COI protein
Haplotypes
Hemiptera
Host plants
Infestation
Mitochondria
Mitochondrial DNA
Pests
Phylogeny
Schizaphis graminum
Triticum aestivum
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Summary:The greenbug, Schizaphis graminum (Rondani), is a major pest of wheat worldwide. Based on host plant response to infestation, several biotypes of this pest have been identified and their phylogenetic relationships inferred using the mitochondrial COI gene. In this study, we have sequenced a fragment of 572 bp of COI gene from 10 S. graminum clones collected, over a three-year period, from 4 sites located in the north and south of Tunisia. Alignment of these sequences revealed the presence of 3 distinct haplotypes, that clustered with the agricultural biotypes I, J, E, K and C. Therefore, we suggest that resistance genes Gb3, Gb4, Gb5 and Gb6 in wheat should be the first choice for use in wheat improvement programs, for resistance against greenbug, in Tunisia.
ISSN:1302-0250