Favoring the birth of female puppies after artificial insemination using chilled semen diluted with powdered coconut water (ACP-106c)

• Published in: Theriogenology 2012-06, Vol.77 (9), p.1959-1963
• Main Authors: Uchoa, Daniel Couto, da Silva, Ticiana Franco Pereira, Cardoso, Janaína de Fátima Saraiva, Filho, Antônio Cavalcante Mota, Jucá, Ricardo Parente, Silva, Alexandre Rodrigues, Machado da Silva, Lúcia Daniel
• Format: Article
• Language: English
• Subjects: Animals; Artificial insemination; bitches; Cocos - chemistry; Cold Temperature; Dog; Dogs - physiology; Female; Insemination, Artificial - methods; Insemination, Artificial - veterinary; litter size; Male; males; natural mating; Powdered coconut water; Pregnancy; puppies; semen; Semen - physiology; Semen extender; Sex Preselection - veterinary; Sex ratio; Water - chemistry; whelping
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2011.11.029

The objective was to determine the effect of powdered coconut water extender (ACP-106c) on the proportion of female puppies born. Twenty French Bulldog bitches were subjected to natural mating (NM) and, during the subsequent two estrus periods, were bred by intravaginal artificial insemination (AI), using chilled semen (from the same males) diluted in Tris-egg yolk (AI-Tris) or ACP-106c (AI-ACP-106c). Fresh semen was cooled to 5 °C and maintained at that temperature for 6 h, rewarmed (37 °C for 30 s), and used for AI. Pregnancy and whelping rates following NM were both 100% and were both 90.0% following AI with either extender. Litter size (mean ± SD) was 5.4 ±1.1, 4.7 ± 2.0, and 5.1 ± 2.0 (P > 0.05) for NM, AI-Tris, and AI-ACP-106c, respectively. Furthermore, for these groups, the number of female vs. male puppies born were 2.6 ± 0.6 vs. 2.8 ± 1.0, 2.2 ± 1.0 vs. 2.5 ± 1.1, and 3.4 ± 1.6 vs. 1.8 ± 1.2 (P < 0.05 for AI-ACP-106c only). In conclusion, our hypothesis was supported; AI of semen in ACP-106c extender resulted in a significantly higher proportion of female puppies. Furthermore, this extender yielded acceptable litter size and rates of pregnancy and whelping.