rs4215 SNP in zinc-α2-glycoprotein gene is associated with obesity in Chinese north Han population

• Published in: Gene 2012-06, Vol.500 (2), p.211-215
• Main Authors: Zhu, Hui-Juan, Dong, Cai-Xia, Pan, Hui, Ping, Xiao-Chuan, Li, Nai-Shi, Dai, Yu-Fei, Wang, Lin-Jie, Yang, Hong-Bo, Zhao, Wei-Gang, Gong, Feng-Ying
• Format: Article
• Language: English
• Subjects: Adipokines - genetics; Adult; Asian Continental Ancestry Group - genetics; Association study; Base Sequence; Body Weight - genetics; Body Weights and Measures; Case-Control Studies; Female; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Humans; Linkage Disequilibrium; Male; Middle Aged; Molecular Sequence Data; Obesity; Obesity - genetics; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide - genetics; Seminal Plasma Proteins - genetics; Sequence Analysis, DNA; Single nucleotide polymorphism (SNP); Zinc-α2-glycoprotein (ZAG)
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2012.03.020

Zinc-α2-glycoprotein (ZAG) has been identified recently as a novel adipokine due to its close link with lipid and glucose metabolism, as well as regulation of body weight. The aim of our present study is to investigate the ZAG genetic polymorphism association with obesity in Chinese north Han population. Five SNPs of ZAG gene including rs2247607 (A>T), rs4727442 (G>T), rs4215 (A>G), rs2527923 (C>T) and rs2527882 (C>T) were genotyped in 648 overweight/obese patients and 313 healthy controls by TaqMan-PCR methods. Crosstabs statistical analysis method with subjects stratifying by age (≦30y, 31–45y, ≧46y) and gender was used. The results showed the constitution of three genotype frequencies in rs4215 (A>G) site significantly differs in male subgroup (aged 31–45y) between overweight/obese and healthy control group (χ2=6.401, P=0.041). GG genotype frequency in overweight/obese group is 19.3% which is much higher than 6.1% in healthy control group. Further statistical analysis under a recessive inheritance model demonstrated odd ratio (OR) for GG vs. AA+AG in overweight/obese group was 3.674 (95% CI 1.049–12.866; P=0.035). Among three genotypes of rs4215, the subjects with GG genotype have much more higher body weight, BMI, waist circumference and SBP. Our data, for the first time, suggest the genotypes of rs4215 in ZAG gene are significantly associated with obesity in Chinese north Han population. GG genotype subjects in rs4215 site have an increased susceptibility to obesity when compared with the AA+AG genotype subjects. ► Association of ZAG gene SNPs with obesity was performed in Chinese people. ► GG genotype subjects in rs4215 site have an increased susceptibility to obesity. ► Participants with GG genotype in rs4215 have much higher BW, BMI, WC and SBP.