Molecular cloning of a new wheat calreticulin gene TaCRT1 and expression analysis in plant defense responses and abiotic stress resistance

Calreticulin proteins play essential roles in regulating various metabolic processes and in molecular signal transduction in animals and plants. Using homologous PCR, we screened a cDNA library of the wheat resistance gene Yr5 from a near-isogenic line in the susceptible common wheat variety Taichun...

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Bibliographic Details
Published in:Genetics and molecular research 2011-11, Vol.10 (4), p.3576-3585
Main Authors: An, Y Q, Lin, R M, Wang, F T, Feng, J, Xu, Y F, Xu, S C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Calreticulin - chemistry
Calreticulin - genetics
Calreticulin - metabolism
Cloning, Molecular
Cluster Analysis
Disease Resistance - genetics
Disease Resistance - immunology
Gene Expression Regulation, Plant - immunology
Genes, Plant - genetics
Molecular Sequence Data
Phylogeny
Plant Diseases - genetics
Plant Diseases - immunology
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Sequence Analysis, DNA
Stress, Physiological - genetics
Stress, Physiological - immunology
Triticum - genetics
Triticum - immunology
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Summary:Calreticulin proteins play essential roles in regulating various metabolic processes and in molecular signal transduction in animals and plants. Using homologous PCR, we screened a cDNA library of the wheat resistance gene Yr5 from a near-isogenic line in the susceptible common wheat variety Taichung 29, which was inoculated with an incompatible race CYR32 of Puccinia striiformis. We isolated a novel full-length cDNA encoding calreticulin protein, which we named TaCRT1. Sequence analyses indicated that TaCRT1 contains an open reading frame of 1287 bp in length; it was deduced to encode 428 amino acids. Clustering analysis showed that TaCRT1 belongs to group III of the calreticulin protein family. Semi-quantitative RT-PCR was used to analyze expression profiles of the isolated gene under biotic and abiotic stresses. Expression of TaCRT1 was suppressed by exogenous application of phytohormones, such as abscisic acid and methyl jasmonate, and by dehydration; but it was induced by CYR32 infection and cold treatment. Based on the expression patterns, we propose that TaCRT1 participates in regulatory processes involved in defense responses and stress resistance in wheat.
ISSN:1676-5680
1676-5680
DOI:10.4238/2011.November.10.1