Cohnella plantaginis sp. nov., a novel nitrogen-fixing species isolated from plantain rhizosphere soil

• Published in: Antonie van Leeuwenhoek 2012-06, Vol.102 (1), p.83-89
• Main Authors: Wang, Li-Ying, Chen, San-Feng, Wang, Lei, Zhou, Yu-Guang, Liu, Hong-Can
• Format: Article
• Language: English
• Subjects: Aerobiosis; Agronomy. Soil science and plant productions; Bacillales - classification; Bacillales - genetics; Bacillales - isolation & purification; Bacillales - physiology; Bacteria; Bacterial Typing Techniques; Base Composition; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; China; Cluster Analysis; Cytosol - chemistry; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Economic plant physiology; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Genotype & phenotype; Life Sciences; Lipids; Locomotion; Medical Microbiology; Microbiology; Molecular Sequence Data; Nitrogen; Nitrogen - metabolism; Nitrogen Fixation; Nucleic Acid Hybridization; Original Paper; Phospholipids - analysis; Phylogeny; Plant Sciences; Plantago - microbiology; Quinones - analysis; Rhizosphere; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Soil microorganisms; Soil Science & Conservation; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-012-9715-1

A novel bacterium capable of fixing nitrogen was isolated from plantain rhizosphere soil in China. The isolate, designated YN-83 T , is Gram-positive, aerobic, motile and rod-shaped (0.4–0.6 μm × 1.9–2.6 μm). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain YN-83 T was a member of the genus Cohnella . High similarity of 16S rRNA gene sequence was found between YN-83 T and Cohnella ginsengisoli DSM18997 T (97.99%), whereas the similarity was below 96.0% between YN-83 T and the other Cohnella species. DNA–DNA relatedness between strain YN-83 T and C. ginsengisoli DSM18997 T was 27.4 ± 6.2%. The DNA G+C content of strain YN-83 T was 59.3 mol%. The predominant isoprenoid quinone was MK-7 and the major fatty acids were anteiso-C 15:0 (44.3%), iso-C 15:0 (11.3%), iso-C 16:0 (18.6%) and C 16:0 (7.7%). The polar lipids of strain YN-83 T consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lyso- phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence, G+C content and DNA–DNA hybridization, strain YN-83 T represents a novel species of the genus Cohnella , for which the name Cohnella plantaginis sp. nov. (type strain YN-83 T  = DSM 25424 T  = CGMCC 1.12047 T ) is proposed.