Application of ex vivo micro-computed tomography for assessment of in vivo fluorescence and plain radiographic imaging for monitoring bone metastases and osteolytic lesions

The intracardiac injection model is a commonly used in vivo model to test therapeutic response in bone metastases. However, few studies have critically compared the performance of different imaging methods in terms of sensitivity and quantitative assessment of osteolytic lesions. We performed in viv...

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Bibliographic Details
Published in:Journal of bone and mineral metabolism 2012-05, Vol.30 (3), p.373-380
Main Authors: Tiwari, Sanjay, Schem, Christian, Lorenzen, Ann-Christin, Kayser, Ole, Wiese, Claas, Graeff, Christian, Peña, Jaime, Marshall, Robert P., Heller, Martin, Kalthoff, Holger, Jonat, Walter, Glüer, Claus-C
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bone Neoplasms - diagnostic imaging
Bone Neoplasms - pathology
Bone Neoplasms - secondary
Cell Line, Tumor
Diseases of the osteoarticular system
Endpoint Determination
Female
Fluorescence
Humans
Luminescent Proteins - metabolism
Medical sciences
Medicine
Medicine & Public Health
Metabolic Diseases
Mice
Mice, Inbred BALB C
Orthopedics
Osteolysis - diagnostic imaging
Osteolysis - pathology
Short Communication
Staining and Labeling
Tumors of striated muscle and skeleton
X-Ray Microtomography - methods
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Summary:The intracardiac injection model is a commonly used in vivo model to test therapeutic response in bone metastases. However, few studies have critically compared the performance of different imaging methods in terms of sensitivity and quantitative assessment of osteolytic lesions. We performed in vivo optical and plain radiographic imaging of bone metastases followed by high-sensitivity ex vivo micro-computed tomography (micro-CT) imaging. This approach allowed for quantitative assessment of in vivo imaging techniques using fluorescence and plain radiography. Comparison of lesions detected in vivo by fluorescent optical imaging with ex vivo micro-CT revealed that the limited spatial resolution of fluorescent optical imaging may underestimate the number of bone metastases. Radiography was compared with micro-CT for the detection of osteolytic lesions. When using dichotomous yes/no grading, there was a 64% agreement in detection of osteolytic lesions. When subjective semiquantitative grading methods were used to assess the extent of osteolytic lesions, a positive association between the micro-CT grades and the square root of the radiography-based grades was observed ( p  
ISSN:0914-8779
1435-5604
DOI:10.1007/s00774-011-0335-z