Detection of Listeria monocytogenes in ready-to-eat food by Step One real-time polymerase chain reaction

• Published in: Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Pesticides, food contaminants, and agricultural wastes, 2012-03, Vol.47 (3), p.212-216
• Main Authors: Pochop, Jaroslav, Kačániová, Miroslava, Hleba, Lukáš, Lopasovský, L'Ubomír, Bobková, Alica, Zeleňáková, Lucia, Stričík, Michal
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; Animals; Applied ecology; Bacteria; Biological and medical sciences; Contamination; Dairy Products - microbiology; detection kit; DNA; DNA, Bacterial - analysis; DNA, Bacterial - isolation & purification; Food; Food Microbiology - instrumentation; Food Microbiology - methods; Fundamental and applied biological sciences. Psychology; Listeria monocytogenes; Listeria monocytogenes - isolation & purification; Meat - microbiology; meat products; milk; Polymerase chain reaction; quantitative polymerase chain reaction; ready-to-eat food; ready-to-eat foods; Reagent Kits, Diagnostic; Real-time PCR; Real-Time Polymerase Chain Reaction - instrumentation; Real-Time Polymerase Chain Reaction - methods; Salmon - microbiology; Seafood - microbiology; Sensitivity and Specificity; Sheep - microbiology
• ISSN: 1532-4109; 0360-1234; 1532-4109
• DOI: 10.1080/03601234.2012.634353

The aim of this study was to follow contamination of ready-to-eat food with Listeria monocytogenes by using the Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Listeria monocytogenes Detection Kit for the real-time PCR performance. In 30 samples of ready-to-eat milk and meat products without incubation we detected strains of Listeria monocytogenes in five samples (swabs). Internal positive control (IPC) was positive in all samples. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in ready-to-eat food without incubation.