Characterization of maize allergens — MON810 vs. its non-transgenic counterpart

• Published in: Journal of proteomics 2012-04, Vol.75 (7), p.2027-2037
• Main Authors: Fonseca, Cátia, Planchon, Sébastien, Renaut, Jenny, Oliveira, Maria Margarida, Batista, Rita
• Format: Article
• Language: English
• Subjects: 2D-gel electrophoresis; Allergen identification; Allergens; Biological and medical sciences; corn; Development; Developmental stages; Diverse techniques; Food; Fundamental and applied biological sciences. Psychology; Gel electrophoresis; gene expression regulation; genes; Genetically modified food safety; genetically modified foods; Immunoglobulin E; Maize allergy; Molecular and cellular biology; Pollination; Polymerase chain reaction; proteomics; reverse transcriptase polymerase chain reaction; seed development; Seeds; transgenic plants; Trypsin; trypsin inhibitors; Western blot; Western blotting; Zea; Zea mays; Zein
• ISSN: 1874-3919
• DOI: 10.1016/j.jprot.2012.01.005

One of the main concerns about genetically modified foods and their potential impacts on human health is that the introduction of a new/ altered gene may putatively alter the expression of others, namely endogenous allergens. We intended to evaluate, and to compare, using quantitative real time RT-PCR technique, the expression of 5 already known maize allergens (Zea m14, Zea m25, Zea m27kD, 50kD Zein and trypsin inhibitor) in MON 810 vs. its non-transgenic counterpart, throughout seed development (10, 16 and 23days after pollination). We have shown that none of the tested allergen genes presented differential expression, with statistic significance, along all tested seed development stages, in MON810 vs. its conventional counterpart. We have also used bidimensional gel electrophoresis followed by Western blotting with plasma from two maize allergic subjects to characterize their immunologic responses against MON 810 vs. its non-transgenic control. Immunoreactive spots were characterized by MS. We have identified fourteen new IgE-binding proteins present in both transgenic and non-transgenic maize. Expression of five known maize allergens, during seed development, was not significantly different between MON810 vs. its control. Identification of fourteen new potential maize allergens. [Display omitted] ► Expression evaluation of 5 maize allergens (MON810 vs. non-transgenic control). ► Differences in allergens’ expression profiles weren't statistically significant. ► Use of Western blot and MS for new potential maize allergens identification. ► Identification of 14 new potential maize allergens.