Inhibition of Metastasis of Circulating Human Prostate Cancer Cells in the Chick Embryo by an Extracellular Matrix Produced by Foreskin Fibroblasts in Culture

We have previously demonstrated the increased metastatic potential of human prostate cancer circulating tumor cells (CTC), compared to their parental cells, in both orthotopic mouse models and the chick embryo model. In the current study, we asked whether an extracellular matrix (ECM), produced by h...

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Published in:Anticancer research 2012-05, Vol.32 (5), p.1573-1577
Main Authors: MENEN, Rhiana, PINNEY, Emmett, HASSANEIN, Mohamed K, KOLOSTOVA, Katarina, BOBEK, Vladimir, SUETSUGU, Atsushi, NAN ZHANG, BOUVET, Michael, NAUGHTON, Gail K, HOFFMAN, Robert M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cells, Cultured
Chick Embryo
Extracellular Matrix - physiology
Fibroblasts - metabolism
Foreskin - metabolism
Humans
Male
Medical sciences
Neoplasm Metastasis - prevention & control
Neoplastic Cells, Circulating - pathology
Nephrology. Urinary tract diseases
Prostatic Neoplasms - pathology
Tumors
Tumors of the urinary system
Urinary tract. Prostate gland
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container_issue 5
container_start_page 1573
container_title Anticancer research
container_volume 32
creator MENEN, Rhiana
PINNEY, Emmett
HASSANEIN, Mohamed K
KOLOSTOVA, Katarina
BOBEK, Vladimir
SUETSUGU, Atsushi
NAN ZHANG
BOUVET, Michael
NAUGHTON, Gail K
HOFFMAN, Robert M
description We have previously demonstrated the increased metastatic potential of human prostate cancer circulating tumor cells (CTC), compared to their parental cells, in both orthotopic mouse models and the chick embryo model. In the current study, we asked whether an extracellular matrix (ECM), produced by human foreskin fibroblasts in culture, could inhibit PC-3 human prostate cancer CTC metastasis in the chick embryo model. The chorioallantoic membranes (CAM) of 18 chicken embryos were inoculated with either PC-3 human prostate cancer cells or PC-3 CTCs, both stably expressing green fluorescent protein (GFP). Embryos were divided into six groups: PC-3 parental-cell control; PC-3 plus soluble ECM; PC-3 parental cells plus semi-solid ECM; PC-3 CTC control; PC-3 CTC plus soluble ECM, and PC-3 CTC plus semi-solid ECM. Twelve hours following inoculation of the cells, a single dose of 100 μl of either soluble or semi-solid ECM was added to the appropriate group. Embryo brains were removed on day 8 post-inoculation, and were processed for cryosectioning. Imaging was performed on the cryosections using a scanning laser microscope in order to count metastatic foci. PC-3 controls had an average of 11.1 metastatic foci compared to 2.55 in the PC-3 plus soluble ECM group and 2.76 (p
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In the current study, we asked whether an extracellular matrix (ECM), produced by human foreskin fibroblasts in culture, could inhibit PC-3 human prostate cancer CTC metastasis in the chick embryo model. The chorioallantoic membranes (CAM) of 18 chicken embryos were inoculated with either PC-3 human prostate cancer cells or PC-3 CTCs, both stably expressing green fluorescent protein (GFP). Embryos were divided into six groups: PC-3 parental-cell control; PC-3 plus soluble ECM; PC-3 parental cells plus semi-solid ECM; PC-3 CTC control; PC-3 CTC plus soluble ECM, and PC-3 CTC plus semi-solid ECM. Twelve hours following inoculation of the cells, a single dose of 100 μl of either soluble or semi-solid ECM was added to the appropriate group. Embryo brains were removed on day 8 post-inoculation, and were processed for cryosectioning. Imaging was performed on the cryosections using a scanning laser microscope in order to count metastatic foci. PC-3 controls had an average of 11.1 metastatic foci compared to 2.55 in the PC-3 plus soluble ECM group and 2.76 (p&lt;0.0001) in the PC-3 plus semi-solid ECM group (p&lt;0.0001). ECM treatment had even greater efficacy on the CTC cells, with an average of 30.9 metastatic foci in the CTC controls compared to 4.38 in the CTC plus soluble ECM group (p&lt;0.0001) and 4.18 in the CTC plus semi-solid ECM group (p&lt;0.0001). The results demonstrate that reduction of CTC metastatic potential is possible, in this case with an ECM produced by human foreskin fibroblasts in culture.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 22593434</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; Chick Embryo ; Extracellular Matrix - physiology ; Fibroblasts - metabolism ; Foreskin - metabolism ; Humans ; Male ; Medical sciences ; Neoplasm Metastasis - prevention &amp; control ; Neoplastic Cells, Circulating - pathology ; Nephrology. Urinary tract diseases ; Prostatic Neoplasms - pathology ; Tumors ; Tumors of the urinary system ; Urinary tract. 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PC-3 controls had an average of 11.1 metastatic foci compared to 2.55 in the PC-3 plus soluble ECM group and 2.76 (p&lt;0.0001) in the PC-3 plus semi-solid ECM group (p&lt;0.0001). ECM treatment had even greater efficacy on the CTC cells, with an average of 30.9 metastatic foci in the CTC controls compared to 4.38 in the CTC plus soluble ECM group (p&lt;0.0001) and 4.18 in the CTC plus semi-solid ECM group (p&lt;0.0001). The results demonstrate that reduction of CTC metastatic potential is possible, in this case with an ECM produced by human foreskin fibroblasts in culture.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Extracellular Matrix - physiology</subject><subject>Fibroblasts - metabolism</subject><subject>Foreskin - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neoplasm Metastasis - prevention &amp; control</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Tumors</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. 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Urinary tract diseases</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Tumors</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. 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In the current study, we asked whether an extracellular matrix (ECM), produced by human foreskin fibroblasts in culture, could inhibit PC-3 human prostate cancer CTC metastasis in the chick embryo model. The chorioallantoic membranes (CAM) of 18 chicken embryos were inoculated with either PC-3 human prostate cancer cells or PC-3 CTCs, both stably expressing green fluorescent protein (GFP). Embryos were divided into six groups: PC-3 parental-cell control; PC-3 plus soluble ECM; PC-3 parental cells plus semi-solid ECM; PC-3 CTC control; PC-3 CTC plus soluble ECM, and PC-3 CTC plus semi-solid ECM. Twelve hours following inoculation of the cells, a single dose of 100 μl of either soluble or semi-solid ECM was added to the appropriate group. Embryo brains were removed on day 8 post-inoculation, and were processed for cryosectioning. Imaging was performed on the cryosections using a scanning laser microscope in order to count metastatic foci. PC-3 controls had an average of 11.1 metastatic foci compared to 2.55 in the PC-3 plus soluble ECM group and 2.76 (p&lt;0.0001) in the PC-3 plus semi-solid ECM group (p&lt;0.0001). ECM treatment had even greater efficacy on the CTC cells, with an average of 30.9 metastatic foci in the CTC controls compared to 4.38 in the CTC plus soluble ECM group (p&lt;0.0001) and 4.18 in the CTC plus semi-solid ECM group (p&lt;0.0001). The results demonstrate that reduction of CTC metastatic potential is possible, in this case with an ECM produced by human foreskin fibroblasts in culture.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>22593434</pmid><tpages>5</tpages></addata></record>
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subjects Animals
Biological and medical sciences
Cells, Cultured
Chick Embryo
Extracellular Matrix - physiology
Fibroblasts - metabolism
Foreskin - metabolism
Humans
Male
Medical sciences
Neoplasm Metastasis - prevention & control
Neoplastic Cells, Circulating - pathology
Nephrology. Urinary tract diseases
Prostatic Neoplasms - pathology
Tumors
Tumors of the urinary system
Urinary tract. Prostate gland
title Inhibition of Metastasis of Circulating Human Prostate Cancer Cells in the Chick Embryo by an Extracellular Matrix Produced by Foreskin Fibroblasts in Culture
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