Study of Luan-Pao-Prescription on ovarian dysfunction in rats

• Published in: Journal of ethnopharmacology 2012-06, Vol.141 (2), p.653-658
• Main Authors: Jiang, Baohong, Sun, Kejia, Li, Ming, Wang, Yanling, Zhuang, Lingzhi, Zhang, Lin, Wang, Yingchun, Liu, Xuan, Wu, Wanying, Guan, Shuhong, Yang, Min, Li, Yuxia, Yu, Jin, Dean-Guo
• Format: Article
• Language: English
• Subjects: Administration, Oral; adrenal glands; adverse effects; Animals; body weight; Chorionic Gonadotropin - pharmacology; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - pharmacology; enzyme-linked immunosorbent assay; estradiol; Estradiol - blood; estrous cycle; Estrous Cycle - drug effects; Female; female fertility; Fertility - drug effects; Fertility Agents, Female - administration & dosage; Fertility Agents, Female - pharmacology; Gonadotropins, Equine - pharmacology; human chorionic gonadotropin; Infertility; Litter Size - drug effects; Luan-Pao-Prescription; Medicine, Chinese Traditional; Ovarian dysfunction; ovaries; Ovary - drug effects; Ovary - metabolism; Ovary - physiopathology; oviducts; Plants, Medicinal; Pregnancy; pregnant mare serum gonadotropin; progeny; Rats; Rats, Sprague-Dawley; spleen; testosterone; Testosterone - blood; Time Factors; uterus
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2011.08.082

Luan-Pao-Prescription (LPP) has been clinically proven to be effective on infertility. In the present study we explored the improvement and underlying mechanism of LPP on ovarian dysfunction in rats. 13month old female rats were randomly divided into 4 groups: the Saline group, the LPP groups treated by low (1.67g/kg), and high-dose (5g/kg) LPP respectively, and the hormone group treated by pregnant mare gonadotrophin serum and chorionic gonadotrophin (PMSG/hCG). The estrous cycle was determined by daily observation of vaginal smears; serum estradiol and testosterone were estimated by enzyme-linked immunosorbent assay; ovarian morphology, ovary volume and fertility of female rats were all detected during the study. During 21days of LPP treatment, about 20% increase of rats with regular estrous cycle of 4–6days was found, but no change was detected on serum estradiol and testosterone at the dose of 1.67g/kg and 5g/kg LPP. Both ovary index and uterus index were up-regulated significantly at the dose of 5g/kg LPP, but no regulation on oviduct index, adrenal gland index, pancreatic gland index and spleen index was observed at the two LPP groups. 5g/kg LPP increased total number of pregnant mothers and the offspring; however there are no offspring in PMSG/hCG group. The offspring exhibited similar body weight in each treatment, and no apparent malformation was found for the cubs. While PMSG/hCG treatment increased the ovary index, serum estradiol and testosterone concentration considerably, but no improvement was found on estrous cycle, oviduct index, uterus index, and reproduction. Administration of LPP may have comparable benefits for ovarian dysfunction, but with fewer side effects. Oral LPP have a better overall influence on rats than PSMG/hCG; it may be more effective in improvement of estrous cycle, ovary function and reproduction.