Variant in the FTO gene and biomarkers related to health in mature Slovak women

• Published in: Anthropologischer Anzeiger 2012-01, Vol.69 (2), p.189-200
• Main Authors: Benčová, Dominika, Siváková, Daniela, Luptáková, Lenka, Cvíčelová, Marta, Michnová, Alexandra
• Format: Article
• Language: English
• Subjects: Adult; Alleles; Alpha-Ketoglutarate-Dependent Dioxygenase FTO; Anthropometric measurements; Biological markers; Biomarkers - analysis; Blood pressure; Blood Pressure - physiology; Body fat; Body Mass Index; Cross-Sectional Studies; Female; fto (rs178 17449) gene; Genetic Variation; Genotype; Genotypes; Humans; menopausal status; Middle Aged; Obesity; Obesity - genetics; Postmenopause; Postmenopause - physiology; Premenopause - physiology; Proteins - genetics; Regression Analysis; slovak women; Slovakia - epidemiology; Womens health
• ISSN: 0003-5548; 2363-7099
• DOI: 10.1127/0003-5548/2012/0173

The purpose of this study was to investigate whether variant (rs178 17449, G/T) in the first intron of the fat mass and obesity-associated gene (FTO) was related to different obesity parameters and blood pressure in mature women from Slovakia. A total of 419 Slovak women (241 premenopausal and 178 postmenopausal) ranging in age from 39 to 65 years were recruited from different parts of Slovakia. The subgroups were categorized based on the WHO (1996) criteria. All participants gave written informed consent for participation in this study. Anthropométrie parameters were measured using standard methods. Fat mass was examined by bioimpedance and blood pressure was measured in the morning during the medical examination. Genomic DNA was extracted from blood or saliva samples by the JETQUICK Tissue DNA spine kit. The FTO variant was determined by PCR and restriction analysis according to the methodology of Hubacek et al. (2008). The obtained data were statistically analyzed by SPSS 17.0 for Windows. The FTO genotype and alíele frequencies in the entire sample and in subgroups according to their menopausal and blood pressure status fell within the Hardy-Weinberg equilibrium. In postmenopausal women the FTO (rsl78 17449) genotype was significantly associated with systolic blood pressure (SBP) (p = 0.024) in the dominant GG/GT vs. TT model and with diastolic blood pressure (DBP) (p = 0.030) in the recessive GG vs. GT/TT and the additive model (p = 0.043), respectively. In these postmenopausal women regression analysis showed a statistically significant effect of age, BMI and FTO dominant model on SBP, and of BMI on DBP among the other variables capable of inducing blood pressure differences. This study demonstrates that the SNP rs178 17449 in the FTO gene is associated with systolic and diastolic blood pressure but not with BMI and obesity variables, as already replicated in several populations throughout the world.