Evaluation of cytotoxicity and degree of conversion of glass ionomer cements reinforced with resin

The objective of the present study was to evaluate the cytotoxicity and degree of monomer conversion of resin-reinforced glass ionomer cements (RGIC) over different time periods. Four RGICs: Fuji Ortho LC (FOLC), Fuji Ortho Band (FOB), Orthoglass (OGL), and Multicure Glass Ionomer (MCI) were evaluat...

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Bibliographic Details
Published in:European journal of orthodontics 2012-06, Vol.34 (3), p.362-366
Main Authors: dos Santos, Rogério L, Pithon, Matheus Melo, Martins, Fernanda O, Romanos, Maria Teresa V, Ruellas, Antônio Carlos O
Format: Article
Language:English
Subjects:
Acrylic Resins
Animals
Cell Survival - drug effects
Dentistry
Glass Ionomer Cements - chemistry
Glass Ionomer Cements - toxicity
L Cells (Cell Line)
Mice
Polymerization
Spectrophotometry, Infrared
Time Factors
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Summary:The objective of the present study was to evaluate the cytotoxicity and degree of monomer conversion of resin-reinforced glass ionomer cements (RGIC) over different time periods. Four RGICs: Fuji Ortho LC (FOLC), Fuji Ortho Band (FOB), Orthoglass (OGL), and Multicure Glass Ionomer (MCI) were evaluated for cytotoxicity in fibroblastic L929 cells and for their degree of monomer conversion over different time periods. Three control groups were also analysed: positive control (C+), consisting of Tween 80 cell detergent; negative control (C-), consisting of phosphate-buffered saline; and cell control (CC), consisting of cells exposed to any material. To evaluate the cytotoxicity, the dye-uptake technique was used and the degree of conversion was evaluated using infrared spectroscopy. The data obtained were analysed by analysis of variance and the Tukey's test. The results showed cytotoxicity of the RGICs at 1 and 24 hours; the viability values of these materials were statistically different from the C- and CC groups (P < 0.05). After 48 hours, the FOLC group was statistically similar to the CC and C- groups but different from the others. At 1 hour, there was no difference in the degree of conversion between the FOLC and OGL groups (P > 0.05) or between the FOB and MCI (P < 0.05) groups. However, at 48 hours, the FOLC group had greater conversion values than the other groups (P < 0.05). There is a direct relationship between the degree of conversion and RGIC cytotoxicity. Following initial polymerization, cytotoxicity decreases and, consequently, the degree of conversion of the material increases.
ISSN:0141-5387
1460-2210
DOI:10.1093/ejo/cjr009