Loading…

Functional TRPV4 channels are expressed in mouse skeletal muscle and can modulate resting Ca2+ influx and muscle fatigue

Skeletal muscle contraction is basically controlled by Ca 2+ release and its reuptake into the sarcoplasmic reticulum. However, the long-term maintenance of muscle function requires an additional Ca 2+ influx from extracellular. Several mechanisms seem to contribute to the latter process, such as st...

Full description

Saved in:
Bibliographic Details
Published in:Pflügers Archiv 2011-01, Vol.461 (1), p.115-122
Main Authors: Pritschow, Bernd W., Lange, Thom, Kasch, Joachim, Kunert-Keil, Christiane, Liedtke, Wolfgang, Brinkmeier, Heinrich
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-p184t-9d92a42cb7a00f0d3660e79122530e5d9d87c70a089bb51e37bccd709602663c3
container_end_page 122
container_issue 1
container_start_page 115
container_title Pflügers Archiv
container_volume 461
creator Pritschow, Bernd W.
Lange, Thom
Kasch, Joachim
Kunert-Keil, Christiane
Liedtke, Wolfgang
Brinkmeier, Heinrich
description Skeletal muscle contraction is basically controlled by Ca 2+ release and its reuptake into the sarcoplasmic reticulum. However, the long-term maintenance of muscle function requires an additional Ca 2+ influx from extracellular. Several mechanisms seem to contribute to the latter process, such as store-operated Ca 2+ entry, stretch-activated Ca 2+ influx and resting Ca 2+ influx. Candidate channels that may control Ca 2+ influx into muscle fibers are the STIM proteins, Orai, and the members of the transient receptor potential (TRP) family of cation channels. Here we show that TRPV4, an osmo-sensitive cation channel of the vanilloid subfamily of TRP channels is functionally expressed in mouse skeletal muscle. Western blot analysis showed the presence of TRPV4-specific bands at about 85 and 100 kDa in all tested muscles. The bands were absent when muscle proteins from TRPV4 deficient mice were analyzed. Using the manganese quench technique, we studied the resting influx of divalent cations into isolated wild-type muscle fibers. The specific TRPV4-channel activator 4α-phorbol-12,13-didecanoate (4α-PDD) stimulated resting influx by about 60% only in wild-type fibers. Electrical stimulation of soleus muscles did not reveal changes in isometric twitch contractions upon application of 4α-PDD, but tetanic contractions (at 120 Hz) were slightly increased by about 15%. When soleus muscles were stimulated with a fatigue protocol, muscle fatigue was significantly attenuated in the presence of 4α-PDD. The latter effect was not observed with muscles from TRPV4 −/− mice. We conclude that TRPV4 is functionally expressed in mouse skeletal muscle and that TRPV4 activation modulates resting Ca 2+ influx and muscle fatigue.
doi_str_mv 10.1007/s00424-010-0883-4
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1017955378</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1017955378</sourcerecordid><originalsourceid>FETCH-LOGICAL-p184t-9d92a42cb7a00f0d3660e79122530e5d9d87c70a089bb51e37bccd709602663c3</originalsourceid><addsrcrecordid>eNo1kUtPwzAQhC0EgvL4AVyQj0gosH4kTo6o4iUhgRBwtRx7WwKOW-JYKv8el5bTHuab0e4OIacMLhmAuooAkssCGBRQ16KQO2TCpOAFByZ2yQRAsKJSVX1ADmP8BAAua75PDjg0XFYAE7K6TcGO3SIYT19fnt8ltR8mBPSRmgEprpYDxoiOdoH2ixSRxi_0OGa8T9F6pCY4as1adcmbEWk2jF2Y06nhF9k282n1B235mRm7ecJjsjczPuLJdh6Rt9ub1-l98fh09zC9fiyWrJZj0biGG8ltqwzADJyoKkDVMM5LAVi6xtXKKjBQN21bMhSqtdYpaCrgVSWsOCLnm9zlsPhOeTXdd9Gi9yZgvkczYKopS6HqjJ5t0dT26PRy6Hoz_Oj_b2WAb4CYpTDHQX8u0pBft47R60r0phKdK9HrSrQUv7pte-Y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1017955378</pqid></control><display><type>article</type><title>Functional TRPV4 channels are expressed in mouse skeletal muscle and can modulate resting Ca2+ influx and muscle fatigue</title><source>Springer Nature</source><creator>Pritschow, Bernd W. ; Lange, Thom ; Kasch, Joachim ; Kunert-Keil, Christiane ; Liedtke, Wolfgang ; Brinkmeier, Heinrich</creator><creatorcontrib>Pritschow, Bernd W. ; Lange, Thom ; Kasch, Joachim ; Kunert-Keil, Christiane ; Liedtke, Wolfgang ; Brinkmeier, Heinrich</creatorcontrib><description>Skeletal muscle contraction is basically controlled by Ca 2+ release and its reuptake into the sarcoplasmic reticulum. However, the long-term maintenance of muscle function requires an additional Ca 2+ influx from extracellular. Several mechanisms seem to contribute to the latter process, such as store-operated Ca 2+ entry, stretch-activated Ca 2+ influx and resting Ca 2+ influx. Candidate channels that may control Ca 2+ influx into muscle fibers are the STIM proteins, Orai, and the members of the transient receptor potential (TRP) family of cation channels. Here we show that TRPV4, an osmo-sensitive cation channel of the vanilloid subfamily of TRP channels is functionally expressed in mouse skeletal muscle. Western blot analysis showed the presence of TRPV4-specific bands at about 85 and 100 kDa in all tested muscles. The bands were absent when muscle proteins from TRPV4 deficient mice were analyzed. Using the manganese quench technique, we studied the resting influx of divalent cations into isolated wild-type muscle fibers. The specific TRPV4-channel activator 4α-phorbol-12,13-didecanoate (4α-PDD) stimulated resting influx by about 60% only in wild-type fibers. Electrical stimulation of soleus muscles did not reveal changes in isometric twitch contractions upon application of 4α-PDD, but tetanic contractions (at 120 Hz) were slightly increased by about 15%. When soleus muscles were stimulated with a fatigue protocol, muscle fatigue was significantly attenuated in the presence of 4α-PDD. The latter effect was not observed with muscles from TRPV4 −/− mice. We conclude that TRPV4 is functionally expressed in mouse skeletal muscle and that TRPV4 activation modulates resting Ca 2+ influx and muscle fatigue.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/s00424-010-0883-4</identifier><identifier>PMID: 20924600</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Calcium ; Calcium (extracellular) ; Calcium - metabolism ; Calcium influx ; cation channels ; Cell Biology ; Electric Stimulation ; Electrical stimuli ; Fatigue ; Human Physiology ; Manganese ; Mice ; Molecular Medicine ; Muscle contraction ; Muscle Contraction - drug effects ; Muscle Fatigue - drug effects ; Muscle Fatigue - physiology ; Muscle Physiology ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - metabolism ; Neurosciences ; Phorbol Esters - pharmacology ; Receptors ; Sarcoplasmic reticulum ; Skeletal muscle ; transient receptor potential proteins ; TRPV Cation Channels - biosynthesis ; Western blotting</subject><ispartof>Pflügers Archiv, 2011-01, Vol.461 (1), p.115-122</ispartof><rights>Springer-Verlag 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p184t-9d92a42cb7a00f0d3660e79122530e5d9d87c70a089bb51e37bccd709602663c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20924600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pritschow, Bernd W.</creatorcontrib><creatorcontrib>Lange, Thom</creatorcontrib><creatorcontrib>Kasch, Joachim</creatorcontrib><creatorcontrib>Kunert-Keil, Christiane</creatorcontrib><creatorcontrib>Liedtke, Wolfgang</creatorcontrib><creatorcontrib>Brinkmeier, Heinrich</creatorcontrib><title>Functional TRPV4 channels are expressed in mouse skeletal muscle and can modulate resting Ca2+ influx and muscle fatigue</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch - Eur J Physiol</addtitle><addtitle>Pflugers Arch</addtitle><description>Skeletal muscle contraction is basically controlled by Ca 2+ release and its reuptake into the sarcoplasmic reticulum. However, the long-term maintenance of muscle function requires an additional Ca 2+ influx from extracellular. Several mechanisms seem to contribute to the latter process, such as store-operated Ca 2+ entry, stretch-activated Ca 2+ influx and resting Ca 2+ influx. Candidate channels that may control Ca 2+ influx into muscle fibers are the STIM proteins, Orai, and the members of the transient receptor potential (TRP) family of cation channels. Here we show that TRPV4, an osmo-sensitive cation channel of the vanilloid subfamily of TRP channels is functionally expressed in mouse skeletal muscle. Western blot analysis showed the presence of TRPV4-specific bands at about 85 and 100 kDa in all tested muscles. The bands were absent when muscle proteins from TRPV4 deficient mice were analyzed. Using the manganese quench technique, we studied the resting influx of divalent cations into isolated wild-type muscle fibers. The specific TRPV4-channel activator 4α-phorbol-12,13-didecanoate (4α-PDD) stimulated resting influx by about 60% only in wild-type fibers. Electrical stimulation of soleus muscles did not reveal changes in isometric twitch contractions upon application of 4α-PDD, but tetanic contractions (at 120 Hz) were slightly increased by about 15%. When soleus muscles were stimulated with a fatigue protocol, muscle fatigue was significantly attenuated in the presence of 4α-PDD. The latter effect was not observed with muscles from TRPV4 −/− mice. We conclude that TRPV4 is functionally expressed in mouse skeletal muscle and that TRPV4 activation modulates resting Ca 2+ influx and muscle fatigue.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Calcium</subject><subject>Calcium (extracellular)</subject><subject>Calcium - metabolism</subject><subject>Calcium influx</subject><subject>cation channels</subject><subject>Cell Biology</subject><subject>Electric Stimulation</subject><subject>Electrical stimuli</subject><subject>Fatigue</subject><subject>Human Physiology</subject><subject>Manganese</subject><subject>Mice</subject><subject>Molecular Medicine</subject><subject>Muscle contraction</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Fatigue - drug effects</subject><subject>Muscle Fatigue - physiology</subject><subject>Muscle Physiology</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Neurosciences</subject><subject>Phorbol Esters - pharmacology</subject><subject>Receptors</subject><subject>Sarcoplasmic reticulum</subject><subject>Skeletal muscle</subject><subject>transient receptor potential proteins</subject><subject>TRPV Cation Channels - biosynthesis</subject><subject>Western blotting</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNo1kUtPwzAQhC0EgvL4AVyQj0gosH4kTo6o4iUhgRBwtRx7WwKOW-JYKv8el5bTHuab0e4OIacMLhmAuooAkssCGBRQ16KQO2TCpOAFByZ2yQRAsKJSVX1ADmP8BAAua75PDjg0XFYAE7K6TcGO3SIYT19fnt8ltR8mBPSRmgEprpYDxoiOdoH2ixSRxi_0OGa8T9F6pCY4as1adcmbEWk2jF2Y06nhF9k282n1B235mRm7ecJjsjczPuLJdh6Rt9ub1-l98fh09zC9fiyWrJZj0biGG8ltqwzADJyoKkDVMM5LAVi6xtXKKjBQN21bMhSqtdYpaCrgVSWsOCLnm9zlsPhOeTXdd9Gi9yZgvkczYKopS6HqjJ5t0dT26PRy6Hoz_Oj_b2WAb4CYpTDHQX8u0pBft47R60r0phKdK9HrSrQUv7pte-Y</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Pritschow, Bernd W.</creator><creator>Lange, Thom</creator><creator>Kasch, Joachim</creator><creator>Kunert-Keil, Christiane</creator><creator>Liedtke, Wolfgang</creator><creator>Brinkmeier, Heinrich</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope></search><sort><creationdate>20110101</creationdate><title>Functional TRPV4 channels are expressed in mouse skeletal muscle and can modulate resting Ca2+ influx and muscle fatigue</title><author>Pritschow, Bernd W. ; Lange, Thom ; Kasch, Joachim ; Kunert-Keil, Christiane ; Liedtke, Wolfgang ; Brinkmeier, Heinrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p184t-9d92a42cb7a00f0d3660e79122530e5d9d87c70a089bb51e37bccd709602663c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Calcium</topic><topic>Calcium (extracellular)</topic><topic>Calcium - metabolism</topic><topic>Calcium influx</topic><topic>cation channels</topic><topic>Cell Biology</topic><topic>Electric Stimulation</topic><topic>Electrical stimuli</topic><topic>Fatigue</topic><topic>Human Physiology</topic><topic>Manganese</topic><topic>Mice</topic><topic>Molecular Medicine</topic><topic>Muscle contraction</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Fatigue - drug effects</topic><topic>Muscle Fatigue - physiology</topic><topic>Muscle Physiology</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Neurosciences</topic><topic>Phorbol Esters - pharmacology</topic><topic>Receptors</topic><topic>Sarcoplasmic reticulum</topic><topic>Skeletal muscle</topic><topic>transient receptor potential proteins</topic><topic>TRPV Cation Channels - biosynthesis</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pritschow, Bernd W.</creatorcontrib><creatorcontrib>Lange, Thom</creatorcontrib><creatorcontrib>Kasch, Joachim</creatorcontrib><creatorcontrib>Kunert-Keil, Christiane</creatorcontrib><creatorcontrib>Liedtke, Wolfgang</creatorcontrib><creatorcontrib>Brinkmeier, Heinrich</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pritschow, Bernd W.</au><au>Lange, Thom</au><au>Kasch, Joachim</au><au>Kunert-Keil, Christiane</au><au>Liedtke, Wolfgang</au><au>Brinkmeier, Heinrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional TRPV4 channels are expressed in mouse skeletal muscle and can modulate resting Ca2+ influx and muscle fatigue</atitle><jtitle>Pflügers Archiv</jtitle><stitle>Pflugers Arch - Eur J Physiol</stitle><addtitle>Pflugers Arch</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>461</volume><issue>1</issue><spage>115</spage><epage>122</epage><pages>115-122</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><abstract>Skeletal muscle contraction is basically controlled by Ca 2+ release and its reuptake into the sarcoplasmic reticulum. However, the long-term maintenance of muscle function requires an additional Ca 2+ influx from extracellular. Several mechanisms seem to contribute to the latter process, such as store-operated Ca 2+ entry, stretch-activated Ca 2+ influx and resting Ca 2+ influx. Candidate channels that may control Ca 2+ influx into muscle fibers are the STIM proteins, Orai, and the members of the transient receptor potential (TRP) family of cation channels. Here we show that TRPV4, an osmo-sensitive cation channel of the vanilloid subfamily of TRP channels is functionally expressed in mouse skeletal muscle. Western blot analysis showed the presence of TRPV4-specific bands at about 85 and 100 kDa in all tested muscles. The bands were absent when muscle proteins from TRPV4 deficient mice were analyzed. Using the manganese quench technique, we studied the resting influx of divalent cations into isolated wild-type muscle fibers. The specific TRPV4-channel activator 4α-phorbol-12,13-didecanoate (4α-PDD) stimulated resting influx by about 60% only in wild-type fibers. Electrical stimulation of soleus muscles did not reveal changes in isometric twitch contractions upon application of 4α-PDD, but tetanic contractions (at 120 Hz) were slightly increased by about 15%. When soleus muscles were stimulated with a fatigue protocol, muscle fatigue was significantly attenuated in the presence of 4α-PDD. The latter effect was not observed with muscles from TRPV4 −/− mice. We conclude that TRPV4 is functionally expressed in mouse skeletal muscle and that TRPV4 activation modulates resting Ca 2+ influx and muscle fatigue.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>20924600</pmid><doi>10.1007/s00424-010-0883-4</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0031-6768
ispartof Pflügers Archiv, 2011-01, Vol.461 (1), p.115-122
issn 0031-6768
1432-2013
language eng
recordid cdi_proquest_miscellaneous_1017955378
source Springer Nature
subjects Animals
Biomedical and Life Sciences
Biomedicine
Calcium
Calcium (extracellular)
Calcium - metabolism
Calcium influx
cation channels
Cell Biology
Electric Stimulation
Electrical stimuli
Fatigue
Human Physiology
Manganese
Mice
Molecular Medicine
Muscle contraction
Muscle Contraction - drug effects
Muscle Fatigue - drug effects
Muscle Fatigue - physiology
Muscle Physiology
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Neurosciences
Phorbol Esters - pharmacology
Receptors
Sarcoplasmic reticulum
Skeletal muscle
transient receptor potential proteins
TRPV Cation Channels - biosynthesis
Western blotting
title Functional TRPV4 channels are expressed in mouse skeletal muscle and can modulate resting Ca2+ influx and muscle fatigue
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T04%3A10%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20TRPV4%20channels%20are%20expressed%20in%20mouse%20skeletal%20muscle%20and%20can%20modulate%20resting%20Ca2+%20influx%20and%20muscle%20fatigue&rft.jtitle=Pfl%C3%BCgers%20Archiv&rft.au=Pritschow,%20Bernd%20W.&rft.date=2011-01-01&rft.volume=461&rft.issue=1&rft.spage=115&rft.epage=122&rft.pages=115-122&rft.issn=0031-6768&rft.eissn=1432-2013&rft_id=info:doi/10.1007/s00424-010-0883-4&rft_dat=%3Cproquest_pubme%3E1017955378%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p184t-9d92a42cb7a00f0d3660e79122530e5d9d87c70a089bb51e37bccd709602663c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1017955378&rft_id=info:pmid/20924600&rfr_iscdi=true