Determination of the population genetic structure of the invasive weed Ageratina adenophora using ISSR-PCR markers

A genetic analysis of nuclear DNA was performed by inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) technique on 32 populations of Ageratina adenophora, an invasive triploid weed in China. Among the 100 ISSR markers detected, 12 showed genetic variation both within and among the pop...

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Bibliographic Details
Published in:Russian journal of plant physiology 2009-05, Vol.56 (3), p.410-416
Main Authors: Gui, F. R, Wan, F. H, Guo, J. Y
Format: Article
Language:English
Subjects:
Ageratina adenophora
Biomedical and Life Sciences
DNA
Gene polymorphism
Genetic analysis
Genetic distance
Genetic diversity
ISSR-PCR
Life Sciences
Nucleotide sequence
Plant Physiology
Plant Sciences
Population genetics
population structure
Research Papers
Weeds
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Summary:A genetic analysis of nuclear DNA was performed by inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) technique on 32 populations of Ageratina adenophora, an invasive triploid weed in China. Among the 100 ISSR markers detected, 12 showed genetic variation both within and among the populations. Among the 446 amplified bands, 93.5% were found polymorphic. Most individuals (99%) displayed a unique ISSR fingerprint pattern, which yielded a high level of polymorphism (P o = 93.5%) and genetic diversity (Nei's H T = 0.2354). The estimates of population variation, based on ISSR-PCR, were high, as measured by the analysis of molecular variance (AMOVA, F ST = 0.3140), the Wright's F-statistics (G ST = 0.3453), and the Shannon's information index (H sp = 0.3716). AMOVA revealed 68.6% genetic variation within the populations and 91.2% within the provinces. The Mantel test showed that genetic distance was significantly correlated with geographic distance.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443709030157