Effect of NtDCN1 gene activated during embyogenesis on organogenesis in tobacco tissue culture

The role of NtDCN1 gene in organogenesis in the culture of tobacco (Nicotiana tabacum L.) somatic tissues was studied. This gene is specifically expressed in tobacco microspores induced for somatic embryogenesis. This gene knockout resulted in a disturbance of formation and development of embyoids f...

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Bibliographic Details
Published in:Russian journal of plant physiology 2010, Vol.57 (1), p.118-123
Main Authors: Dolgikh, Yu. I, Stepanova, A. Yu, Osipova, E. S, Tashpulatov, A. Sh, Hosp, J, Ribarits, A, Heberle-Bors, E, Touraev, A. M
Format: Article
Language:English
Subjects:
auxin
Auxins
Biomedical and Life Sciences
Hormones
Leaves
Life Sciences
Nicotiana tabacum
NtDCN1 gene
Organogenesis
Plant Physiology
Plant Sciences
Research Papers
Roots
Shoots
Somatic embryogenesis
Tissue culture
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Summary:The role of NtDCN1 gene in organogenesis in the culture of tobacco (Nicotiana tabacum L.) somatic tissues was studied. This gene is specifically expressed in tobacco microspores induced for somatic embryogenesis. This gene knockout resulted in a disturbance of formation and development of embyoids from microspores. In leaf disks and calli derived from tobacco lines with active and inactivated NtDCN1 gene, we studied induction of shoots and roots. A comparative analysis of tobacco line morphogenetic responses in vitro showed that NtDCN1 gene inactivation enhanced shoot formation and suppressed rhizogenesis, whereas this gene reactivation returned organogenesis processes to control level. Difference between lines was manifested only at a definite ratio between exogenous hormones supplied. The involvement of NtDCN1 gene in line responses to exogenous auxin is discussed. The results obtained permit a supposition that the NtDCN1 gene is critical for regulation not only somatic embryogenesis but also organogenesis.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443710010164