Amperometric glucose microbiosensor based on a Prussian Blue modified carbon fiber electrode for physiological applications

Results of experiments in vitro and in vivo, using an amperometric glucose microbiosensor based on a Prussian Blue (PB) modified carbon fiber electrode with very low dimensions (∼10μm diameter), are presented. The electrocatalytic properties of the PB film enable detection of an enzymatic by-product...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2011-03, Vol.152 (2), p.137-143
Main Authors: Salazar, P., O’Neill, R.D., Martín, M., Roche, R., González-Mora, J.L.
Format: Article
Language:English
Subjects:
Biosensor
Biosensors
brain
Brain glucose
carbon
Carbon fiber electrode
dielectric spectroscopy
electrical treatment
electrodes
glucose
hydrogen peroxide
in vitro studies
intraperitoneal injection
Polyphenylenediamine
Prussian blue
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Summary:Results of experiments in vitro and in vivo, using an amperometric glucose microbiosensor based on a Prussian Blue (PB) modified carbon fiber electrode with very low dimensions (∼10μm diameter), are presented. The electrocatalytic properties of the PB film enable detection of an enzymatic by-product (H2O2) at a very low applied potential: 0.0V against SCE. The main steps during glucose microbiosensor construction were examined by cyclic voltammetry and electrochemical impedance spectroscopy. Excellent selectivity of the glucose microbiosensors against a large number of physiological interference compounds is demonstrated. Finally, microbiosensor responses during intraperitoneal injection, local infusion and local electrical stimulation showed sufficient sensitivity and stability to monitor multi-phasic and reversible changes in brain ECF glucose levels during physiological experiments, illustrating the excellent properties and utility of this biosensor design in the neurosciences.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2010.11.056