Effect of menstrual blood-derived stromal stem cells on proliferative capacity of peripheral blood mononuclear cells in allogeneic mixed lymphocyte reaction

• Published in: The journal of obstetrics and gynaecology research 2012-05, Vol.38 (5), p.804-809
• Main Authors: Nikoo, Shohreh, Ebtekar, Massoumeh, Jeddi-Tehrani, Mahmood, Shervin, Adel, Bozorgmehr, Mahmood, Kazemnejad, Somaieh, Zarnani, Amir Hassan
• Format: Article
• Language: English
• Subjects: Adherent cells; Adult; allogeneic mixed lymphocyte reaction; CD105 antigen; CD29 antigen; CD34 antigen; CD38 antigen; CD44 antigen; CD45 antigen; CD73 antigen; CD9 antigen; Cell Proliferation; Cells, Cultured; Coculture Techniques; Differentiation; Female; Flow Cytometry; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - immunology; Humans; Immunomodulation; Immunophenotyping; Immunoregulation; Leukocytes (mononuclear); Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - immunology; Lymphocyte Culture Test, Mixed; menstrual blood; Menstruation; Mesenchyme; Middle Aged; Mixed leukocyte reaction; Monoclonal antibodies; Obstetrics; Peripheral blood mononuclear cells; proliferation; regulatory T cells; Stem cells; Stromal Cells - cytology; Stromal Cells - immunology; stromal stem cells
• ISSN: 1341-8076; 1447-0756
• DOI: 10.1111/j.1447-0756.2011.01800.x

Aim:  Menstrual blood stromal stem cells (MBSCs) have been demonstrated to exhibit stem cell properties such as the capability for self‐renewal and multipotency, allowing for multilineage differentiation. In addition, this cell type has various immunomodulatory effects. In this study, we examined the potential effect of MBSCs on proliferation of peripheral blood mononuclear cells (PBMCs) in allogeneic mixed lymphocyte reaction (MLR). Materials and Methods:  Menstrual blood was collected from healthy donors after menstrual blood flow initiated and its mononuclear cell fraction was separated. Cells were subsequently cultured and adherent cells were allowed to propagate and used as stem cells. Flowcytometric immunophenotyping was performed using a panel of monoclonal antibodies including CD44, CD45, CD34, CD9, CD29, CD10, CD38, CD105, CD73, CD133, STRO‐1 and Oct‐4A. For functional analysis, PBMCs were co‐cultured with MBSCs, collected after 4 days and added to allogeneic PBMCs. 2,3‐Bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide (XTT) assay was carried out to evaluate cell proliferation. Results:  MBSCs showed surface and intracellular markers of mesenchymal stem cells with the exception of the high expression of Oct‐4A. MBSCs affected the proliferative response of PBMC in a dose‐dependent manner. At ratio of 1:1 to 1:2, MBSCs inhibited, while at lower ratios (1:32 to 1:64) stimulated the proliferative capacity of allogeneic PBMCs. Conclusion:  According to the present study, MBSCs exert their immunoregulatory effects on allogeneic PBMCs in a dose‐dependent manner. This finding can be considered as a valuable point in future cell therapy strategies, when this cell population is used.