Compatibility between Calpurnia aurea leaf extract, attraction aggregation, and attachment pheromone and entomopathogenic fungus Metarhizium anisopliae on viability, growth, and virulence of the pathogen

Metarhizium anisopliae sensu stricto (ss) (Metsch.) Sorok. isolate ICIPE 07 is being developed as biopesticide for the control of ticks. In addition, leaf extracts of Calpurnia aurea Benth, and the attraction aggregation and attachment pheromone (AAAP) are being used as ticks’ attractant. The three...

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Bibliographic Details
Published in:Journal of pest science 2012-03, Vol.85 (1), p.109-115
Main Authors: Nana, Paulin, Maniania, Nguya K., Maranga, Rosebella O., Boga, Hamadi I., Kutima, Helen L., Eloff, Jacobus N.
Format: Article
Language:English
Subjects:
Agglomeration
Agriculture
Attractants
Attraction
Bioassays
Biomedical and Life Sciences
Calpurnia aurea
Compatibility
Developmental stages
Ecology
Entomology
Entomopathogenic fungi
Forestry
Fungi
Grazing
Inoculum
Ixodidae
Leaves
Life Sciences
Metarhizium anisopliae
Original Paper
Pathogens
Pesticides
Pests
Pheromones
Plant extracts
Plant Pathology
Plant Sciences
Rhipicephalus appendiculatus
Semiochemicals
Spores
Ticks
Viability
Virulence
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Summary:Metarhizium anisopliae sensu stricto (ss) (Metsch.) Sorok. isolate ICIPE 07 is being developed as biopesticide for the control of ticks. In addition, leaf extracts of Calpurnia aurea Benth, and the attraction aggregation and attachment pheromone (AAAP) are being used as ticks’ attractant. The three agents are being considered for use in combination in an autodissemination approach, whereby ticks that are attracted to semiochemicals are infected with the inoculum. Experiments were therefore conducted to evaluate in vitro the compatibility between C. aurea, AAAP, and the M. anisopliae on vegetative growth, conidial production, and spore viability. Calpurnia aurea leaf extract was compatible with the fungus at all the concentrations tested, whereas AAAP inhibited all the fungal growth parameters. The virulence of M. anisopliae formulated in emulsifiable extracts of C. aurea was also tested against different developmental stages of Rhipicephalus appendiculatus in laboratory bioassays. No significant differences in virulence were observed between M. anisopliae applied alone and M. anisopliae formulated in different concentrations of C. aurea leaf extracts. These results suggest that C. aurea leaf extracts is compatible with M. anisopliae and could be mixed together for “spot-spray” treatments as low-cost and environmental-friendly technology to control ticks in grazing field, while AAAP should be used separately.
ISSN:1612-4758
1612-4766
DOI:10.1007/s10340-011-0399-5