Production of cloned transgenic cow expressing omega-3 fatty acids

n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3...

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Bibliographic Details
Published in:Transgenic research 2012-06, Vol.21 (3), p.537-543
Main Authors: Wu, Xia, Ouyang, Hongsheng, Duan, Biao, Pang, Daxin, Zhang, Li, Yuan, Ting, Xue, Lian, Ni, Daibang, Cheng, Lei, Dong, Shuhua, Wei, Zhuying, Li, Lin, Yu, Ming, Sun, Qing-Yuan, Chen, Da-Yuan, Lai, Liangxue, Dai, Yifan, Li, Guang-Peng
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Animals, Genetically Modified - genetics
Animals, Genetically Modified - metabolism
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biotechnology
Blotting, Southern
Caenorhabditis elegans
Caenorhabditis elegans - genetics
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - metabolism
Cattle
cows
desaturase
Domestic animals
Economics
Embryo Transfer
Fatty Acid Desaturases - genetics
Fatty Acid Desaturases - metabolism
Fatty acids
Fatty Acids, Omega-3 - genetics
Fatty Acids, Omega-3 - metabolism
Female
Fundamental and applied biological sciences. Psychology
genes
Genes, Helminth
Genetic Engineering
Genetic technics
Genetic Vectors - genetics
Genetic Vectors - metabolism
Life Sciences
Methods. Procedures. Technologies
Milk
Milk - metabolism
Molecular Medicine
Nuclear Transfer Techniques
omega-3 fatty acids
Oocytes - cytology
Oocytes - metabolism
Original Paper
Plant Genetics and Genomics
Polyunsaturated fatty acids
Pregnancy
stearoyl-CoA desaturase
tissue analysis
Transfection
Transgenic animals
Transgenic animals and transgenic plants
Transgenics
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Summary:n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P 
ISSN:0962-8819
1573-9368
DOI:10.1007/s11248-011-9554-2