Methothrexate attenuates early neutrophil infiltration and the associated lipid peroxidation in the injured spinal cord but does not induce neurotoxicity in the uninjured spinal cord in rats

Backround The goal of most acute therapies for spinal cord injury (SCI) in humans include attenuation of the early inflammatory response and may limit the extent of tissue injury and the consequent disability. Objective The purpose of this study was to investigate the early effects of methothrexate...

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Bibliographic Details
Published in:Acta neurochirurgica 2012-06, Vol.154 (6), p.1045-1054
Main Authors: Şanlı, Ahmet Metin, Serbes, Gökhan, Sargon, Mustafa F., Çalışkan, Murat, Kılınç, Kamer, Bulut, Hüsamettin, Şekerci, Zeki
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - pharmacology
Anti-Inflammatory Agents - toxicity
Antiinflammatory agents
Axons
Chemotaxis, Leukocyte - drug effects
Chemotaxis, Leukocyte - physiology
Disease Models, Animal
Experimental Research
Female
Immunosuppressive Agents - pharmacology
Immunosuppressive Agents - toxicity
Inflammation
Interventional Radiology
Leukocytes (neutrophilic)
Lipid peroxidation
Lipid Peroxidation - drug effects
Lipid Peroxidation - physiology
Malondialdehyde
Medicine
Medicine & Public Health
Methotrexate - pharmacology
Methotrexate - toxicity
methylprednisolone
Minimally Invasive Surgery
Neurology
Neuroradiology
Neurosurgery
Neurotoxicity
Neutrophil Infiltration - drug effects
Neutrophil Infiltration - immunology
Peroxidase
Rats
Rats, Wistar
sodium succinate
Spinal Cord Injuries - drug therapy
Spinal Cord Injuries - metabolism
Spinal Cord Injuries - pathology
Spinal cord injury
Surgical Orthopedics
Trauma
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Summary:Backround The goal of most acute therapies for spinal cord injury (SCI) in humans include attenuation of the early inflammatory response and may limit the extent of tissue injury and the consequent disability. Objective The purpose of this study was to investigate the early effects of methothrexate (MTX) treatment on myeloperoxidase (MPO) activity, malondialdehyde (MDA) level, and ultrastructural findings in the injured and uninjured spinal cords of rats. The effects of MTX treatment were also compared with methylprednisolone sodium succinate (MPSS) treatment. Methods Winstar rats were divided into seven groups: control; trauma alone (50 g/cm weight drop trauma); SCI + MPSS (30 mg/kg); SCI + low-dose (0.5 mg/kg) MTX (LDMTX); SCI + higher-dose (1 mg/kg) MTX (HDMTX); non-trauma + LDMTX; non-trauma + HDMTX. Results Administration of MTX and MPSS treatments significantly decreased MPO activity ( p  
ISSN:0001-6268
0942-0940
DOI:10.1007/s00701-012-1302-8