Auxin synthesis by the higher fungus Lentinus edodes (Berk.) sing in the presence of low concentrations of indole compounds

• Published in: Applied biochemistry and microbiology 2012-05, Vol.48 (3), p.280-289
• Main Authors: Tsivileva, O. M, Loshchinina, E. A, Makarov, O. E, Nikitina, V. E
• Format: Article
• Language: English
• Subjects: Acetic acid; anthranilic acid; Basidiomycetes; Biochemistry; Biomedical and Life Sciences; Biosynthesis; Fungi; indole acetic acid; Lentinula edodes; Life Sciences; Medical Microbiology; Microbiology; mushrooms; Protein synthesis
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683812010176

The auxin formation in a submerged culture of the xylotrophic basidiomycete Lentinus edodes (Berk.) Sing (Lentinula edodes (Berk.) Pegler) (shiitake) is studied. Biologically active substances of an indole nature are identified, “the effect of small doses” of which lies in not only the stimulation of growth of the mycelium (indole-3-acetic acid, 2 × 10−7–2 × 10−4 g/l), but also in the induction of tryptophan-independent paths of auxin biosynthesis. The above-mentioned path is realized in the presence of exogenous indole (1 × 10−3–1 × 10−4 g/l), as well as while inducing the biosynthesis of indole-3-acetic acid by its microadditives (1 × 10−5−1 × 10−8 g/l), and is accompanied by the formation of anthranilic acid (up to 1.5 mg/l). Induction of the generative development stage of shiitake by indole derivatives is revealed. It was found that among the studied compounds only indoleacetamide at a concentration of an order of ×10−4 g/l in the culture fluid of L. edodes had a pronounced stimulatory effect on the formation of shiitake’s brown mycelial film.