Relationship between aquaporin-5 expression and saliva flow in streptozotocin-induced diabetic mice?

Oral Diseases (2012) 18, 501–505 Objective:  To investigate the expression and distribution of AQP5 in submandibular acinar cells from sham‐ and streptozotocin (STZ)‐treated mice in relation to the salivary flow. Methods:  Mice were sham or STZ injected. Distribution of AQP5 subcellular expression i...

Full description

Saved in:
Bibliographic Details
Published in:Oral diseases 2012-07, Vol.18 (5), p.501-505
Main Authors: Soyfoo, MS, Bolaky, N, Depoortere, I, Delporte, C
Format: Article
Language:English
Subjects:
Acinar Cells - metabolism
Animals
Aquaporin 5 - biosynthesis
Aquaporin 5 - genetics
aquaporin-5
Cell Membrane - chemistry
Dentistry
Diabetes Mellitus, Experimental - chemically induced
Diabetes Mellitus, Experimental - genetics
Diabetes Mellitus, Experimental - physiopathology
Diabetes Mellitus, Type 1 - genetics
Diabetes Mellitus, Type 1 - physiopathology
diabetic mice
Disease Models, Animal
hyperglycaemia
Hyperglycemia - physiopathology
Immune system
inflammatory infiltrates
Male
Mice
Osmolar Concentration
Rodents
Saliva - secretion
salivary flow
Secretory Rate
Streptozocin
Submandibular Gland - physiology
submandibular glands
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oral Diseases (2012) 18, 501–505 Objective:  To investigate the expression and distribution of AQP5 in submandibular acinar cells from sham‐ and streptozotocin (STZ)‐treated mice in relation to the salivary flow. Methods:  Mice were sham or STZ injected. Distribution of AQP5 subcellular expression in submandibular glands was determined by immunohistochemistry. AQP5 labelling indices (LI), reflecting AQP5 subcellular distribution, were determined in acinar cells. Western blotting was performed to determine the expression of AQP5 in submandibular glands. Blood glycaemia and osmolality and saliva flow rates were also determined. Results:  AQP5 immunoreactivity was primarily located at the apical and apical‐basolateral membranes of submandibular gland acinar cells from sham‐ and STZ‐treated mice. No significant differences in AQP5 protein levels were observed between sham‐ and STZ‐treated mice. Compared to sham‐treated mice, STZ‐treated mice had significant increased glycaemia, while no significant differences in blood osmolality were observed. Saliva flow rate was significantly decreased in STZ‐treated mice as compared to sham‐treated mice. Conclusions:  In STZ‐treated mice, significant reduction in salivary flow rate was observed without any concomitant modification in AQP5 expression and localization.
ISSN:1354-523X
1601-0825
DOI:10.1111/j.1601-0825.2011.01902.x