Effect of low temperature on chlorophyll biosynthesis in albinism line of wheat (Triticum aestivum) FA85

• Published in: Physiologia plantarum 2012-07, Vol.145 (3), p.384-394
• Main Authors: Liu, Xiao-Gang, Xu, Hong, Zhang, Jing-Ya, Liang, Guang-Wang, Liu, Ying-Tuan, Guo, Ai-Guang
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cold Temperature; Color; Enzyme Activation; Ferrochelatase - genetics; Ferrochelatase - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; Lyases - genetics; Lyases - metabolism; Oxidoreductases - genetics; Oxidoreductases - metabolism; Phenotype; Plant Leaves - enzymology; Plant Leaves - genetics; Plant Leaves - metabolism; Plant physiology and development; Plant Proteins - genetics; Plant Proteins - metabolism; Protochlorophyllide - biosynthesis; Protochlorophyllide - genetics; Protoporphyrins - biosynthesis; Protoporphyrins - genetics; RNA, Plant - genetics; Species Specificity; Time Factors; Transcription, Genetic; Triticum - enzymology; Triticum - genetics; Triticum - metabolism
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/j.1399-3054.2012.01604.x

The ‘stage albinism line of winter wheat' FA85 exhibits a severe block in chlorophyll (Chl) biosynthesis with prolonged low‐temperature treatment. The correlations between leaf color and low temperature provide more comprehensive understanding of low temperature as an environmental signal that regulate the metabolic changes in the entire Chl‐synthesizing pathway. In this study, we investigated differences in Chl biosynthesis between leaves of Aibian1 and FA85 by measuring their Chl precursors and heme content, transcripts for key genes of Chl biosynthesis and key enzyme activities. With prolonged low‐temperature treatment, the Chl content gradually decreased, but Chl precursors, including protoporphyrin IX, Mg‐protoporphyrin IX and protochlorophyllide (Pchlide), simultaneously accumulated. Parallel to the decline in Chl content, the protoporphyrin IX distribution toward Chl synthesis was less than that in heme synthesis in the leaves of FA85. Corresponding to the change of protoporphyrin IX distribution, the relative changes in magnesium chelatase (EC 6.6.1.1) and ferrochelatase (EC 4.99.1.1) activities in the leaves of FA85 also indirectly reflected channeling of the metabolic flow into heme rather than Chl. A drastic loss in the transcripts for Pchlide oxidoreductase (EC 1.3.1.33) and Chl synthase (EC 2.5.1.62) accounted for a decrease in the metabolic flux and the re‐direction of metabolites. The high‐level accumulations of Chl precursors and traces of Chl in the leaves of FA85 suggest that a severe block between the steps from Pchlide to Chl formation during Chl biosynthesis is partially derived from the transcriptional downregulation of Pchlide oxidoreductase and Chl synthase.