Immobilization of Pholiota adiposa xylanase onto SiO2 nanoparticles and its application for production of xylooligosaccharides

Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k cat value (4,261 s−1) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparati...

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Bibliographic Details
Published in:Biotechnology letters 2012-07, Vol.34 (7), p.1307-1313
Main Authors: Dhiman, Saurabh Sudha, Jagtap, Sujit Sadashiv, Jeya, Marimuthu, Haw, Jung-Rim, Kang, Yun Chan, Lee, Jung-Kul
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Enzymes
Enzymes, Immobilized - metabolism
Fundamental and applied biological sciences. Psychology
Fungi
General aspects
Immobilization techniques
immobilized enzymes
industrial applications
Life Sciences
Materials science
Methods. Procedures. Technologies
Microbiology
Nanoparticles
Oligosaccharides - metabolism
Original Research Paper
Pholiota
Pholiota - enzymology
Protein Binding
Silicon Dioxide - metabolism
xylanases
xylooligosaccharides
Xylosidases - metabolism
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Summary:Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k cat value (4,261 s−1) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparation onto functionalized silicon oxide nanoparticles, 66 % of the loaded enzyme was retained on the particle. Immobilized enzyme gave 45 % higher concentrations of xylooligosaccharides compared to the free enzyme. After 17 cycles, the immobilized enzyme retained 97 % of the original activity, demonstrating its prospects for the synthesis of xylooligosaccharides in industrial applications.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-012-0902-y