DNA Immunization Against Proacrosin Impairs Fertility in Male Mice

Problem Evaluation of proacrosin/acrosin ability to induce an immune response in male mice after genetic immunization and assessment of animal fertility. Method of study Mice received 50 μg per animal of a plasmid containing the human proacrosin cDNA (pSF2‐Acro) (control: empty plasmid, pSF2). The h...

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Bibliographic Details
Published in:American journal of reproductive immunology (1989) 2012-07, Vol.68 (1), p.56-67
Main Authors: García, Lucila, Veiga, María F., Lustig, Livia, Vazquez-Levin, Mónica H., Veaute, Carolina
Format: Article
Language:English
Subjects:
Acrosin - genetics
Acrosin - immunology
Acrosomal exocytosis
Animals
Autoantibodies - immunology
Contraception, Immunologic
Enzyme Precursors - genetics
Enzyme Precursors - immunology
Female
Fertility - immunology
fertilization
genetic immunization
Humans
Immunization
infertility
Male
Mice
Mice, Inbred BALB C
proacrosin/acrosin
sperm antigen
Sperm-Ovum Interactions - immunology
Spermatozoa - immunology
Vaccines, Contraceptive - genetics
Vaccines, Contraceptive - immunology
Vaccines, DNA - genetics
Vaccines, DNA - immunology
zona pellucida
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Summary:Problem Evaluation of proacrosin/acrosin ability to induce an immune response in male mice after genetic immunization and assessment of animal fertility. Method of study Mice received 50 μg per animal of a plasmid containing the human proacrosin cDNA (pSF2‐Acro) (control: empty plasmid, pSF2). The humoral response was evaluated by ELISA and immunocytochemistry. In vivo fertility was assessed by mating immunized males with control females. The effect of antibodies upon Ca+2‐ionophore‐induced acrosomal exocytosis (AE) and in vitro sperm–zona pellucida (ZP) binding was also studied. Results pSF2‐Acro‐immunized mice developed high levels of specific antibodies (P 
ISSN:1046-7408
1600-0897
DOI:10.1111/j.1600-0897.2012.01127.x