The upregulation of tumour necrosis factor-α and surface antigens expression on macrophages by bisphenol A-glycidyl-methacrylate

• Published in: International endodontic journal 2012-07, Vol.45 (7), p.619-626
• Main Authors: Kuan, Y.-H., Li, Y.-C., Huang, F.-M., Chang, Y.-C.
• Format: Article
• Language: English
• Subjects: Animals; Antigens, Surface - biosynthesis; Antigens, Surface - genetics; Bisphenol A-Glycidyl Methacrylate - toxicity; bisphenol A-glycidyl-methacrylate; Cell Line; Cell Survival - drug effects; Dentistry; macrophage; Macrophage Activation - drug effects; Macrophages - drug effects; Macrophages - immunology; Macrophages - metabolism; Major Histocompatibility Complex - drug effects; Mice; surface antigens; TNF-α; Tumor Necrosis Factor-alpha - biosynthesis; Tumor Necrosis Factor-alpha - genetics; Up-Regulation
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2012.02017.x

Kuan Y‐H, Li Y‐C, Huang F‐M, Chang Y‐C. The upregulation of tumour necrosis factor‐α and surface antigens expression on macrophages by bisphenol A‐glycidyl‐methacrylate. International Endodontic Journal, 45, 619–626, 2012. Aim  To evaluate the expression of tumour necrosis factor‐α and surface antigens by bisphenol A‐glycidyl‐methacrylate (BisGMA) on murine macrophage cell line RAW264.7. Methodology  Cytotoxicity was measured by tetrazolium bromide reduction assay. Tumour necrosis factor (TNF)‐α was analysed by enzyme‐linked immunosorbent assay. Cell surface antigens were investigated by flowcytometry. Statistical analyses were performed using anova followed by the Bonferroni’s t‐test for multigroup comparisons. Results  BisGMA exhibited cytotoxicity to RAW264.7 in a dose‐dependent manner (P