Structural analysis of the O-polysaccharide of the lipopolysaccharide from Azospirillum brasilense Jm6B2 containing 3-O-methyl-d-rhamnose (d-acofriose)

• Published in: Carbohydrate research 2012-07, Vol.355, p.92-95
• Main Authors: Boyko, Alevtina S., Dmitrenok, Andrey S., Fedonenko, Yuliya P., Zdorovenko, Evelina L., Konnova, Svetlana A., Knirel, Yuriy A., Ignatov, Vladimir V.
• Format: Article
• Language: English
• Subjects: Azospirillum brasilense; Azospirillum brasilense - chemistry; Azospirillum brasilense - growth & development; Bacterial polysaccharide structure; Carbohydrate Conformation; carbon; d-Acofriose; Hydrolysis; Lipopolysaccharide; lipopolysaccharides; Lipopolysaccharides - chemistry; Lipopolysaccharides - isolation & purification; Mannose - analogs & derivatives; Mannose - chemistry; methylation; nuclear magnetic resonance spectroscopy; O-Antigen; polysaccharides; rhamnose; stable isotopes
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2012.04.006

[Display omitted] ► Polysaccharides were isolated from the Azospirillum brasilense lipopolysaccharide. ► The major polysaccharide was studied by chemical methods and NMR spectroscopy. ► It was found to contain l-fucose, d-xylose and partially 3-O-methylated d-rhamnose. ► A branched trisaccharide-repeat structure of the polysaccharide was established. Two types of neutral O-polysaccharides were obtained by mild acid degradation of the lipopolysaccharide isolated by phenol–water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense Jm6B2. The following structure of the major O-polysaccharide was established by composition and methylation (ethylation) analyses, Smith degradation, and 1D and 2D 1H and 13C NMR spectroscopy: [Display omitted] where a non-stoichiometric (∼60%) 3-O-methylation of d-rhamnose is indicated by italics.