1β-Hydroxy-2-oxopomolic Acid Isolated from Agrimonia pilosa Extract Inhibits Adipogenesis in 3T3-L1 Cells

• Published in: Biological & pharmaceutical bulletin 2012/05/01, Vol.35(5), pp.643-649
• Main Authors: Ahn, Eun-Kyung, Lee, Jung A, Seo, Dong-Wan, Hong, Seong Su, Oh, Joa Sub
• Format: Article
• Language: English
• Subjects: 1β-hydroxy-2-oxopomolic acid; 3T3-L1 cell line; 3T3-L1 Cells; Adipocytes - cytology; Adipocytes - drug effects; Adipocytes - metabolism; adipogenesis; Adipogenesis - drug effects; Adipogenesis - genetics; adipogenic marker gene; Adipokines - genetics; Adipokines - metabolism; Agrimonia - chemistry; Animals; CCAAT-Enhancer-Binding Protein-alpha - metabolism; Chromans - pharmacology; Dose-Response Relationship, Drug; Down-Regulation; Gene Expression - drug effects; Genetic Markers; Hypoglycemic Agents - pharmacology; Lipid Metabolism - drug effects; Lipid Metabolism - genetics; Mice; Plant Extracts - chemistry; Plant Extracts - pharmacology; PPAR gamma - genetics; PPAR gamma - metabolism; Thiazolidinediones - pharmacology; Transcription, Genetic - drug effects; Triterpenes - isolation & purification; Triterpenes - pharmacology; Troglitazone
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.35.643

In order to determine anti-adipogenic effect, this study investigated 1β-hydroxy-2-oxopomolic acid (HOA) isolated from Agrimonia pilosa inhibits adipocyte differentiation and expression of adipogenic marker genes, such as peroxisome proliferator activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα), glucose transporter 4 (GLUT4), adiponectin, adipocyte fatty acid-binding protein 2 (aP2), adipocyte determination and differentiation factor 1/sterol regulatory element binding protein 1c (ADD1/SREBP1c), resistin, and fatty acid synthase (Fas) in 3T3-L1 preadipocyte. We demonstrated that HOA induced a significant decrease in lipid accumulation and expression of adipogenic marker genes in a dose-dependent manner. In addition, HOA reduced the transcripitional activity of PPARγ induced by troglitazone, a potent diabetes agent; it also suppressed expression of PPARγ and C/EBPα protein levels. Our data suggest that HOA isolated from Agrimonia pilosa inhibits adipocyte differentiation through downregulation of various adipocytokines by blocking PPARγ and C/EBPα expression.