Fast-mode duplex qPCR for BCR-ABL1 molecular monitoring: Innovation, automation, and harmonization

Reverse transcription quantitative polymerase chain reaction (RTqPCR)is currently the most sensitive tool available for the routine monitoring of disease level in patients undergoing treatment for BCRABL1 associated malignancies. Considerable effort has been invested at both the local and internatio...

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Bibliographic Details
Published in:American journal of hematology 2012-07, Vol.87 (7), p.717-720
Main Authors: Gerrard, Gareth, Mudge, Katherine, Foskett, Pierre, Stevens, David, Alikian, Mary, White, Helen E., Cross, Nicholas C.P., Apperley, Jane, Foroni, Letizia
Format: Article
Language:English
Subjects:
Automation, Laboratory
Biological and medical sciences
Biomarkers
Diffusion of Innovation
Fusion Proteins, bcr-abl - blood
Fusion Proteins, bcr-abl - genetics
Fusion Proteins, bcr-abl - metabolism
Hematologic and hematopoietic diseases
Hematology
High-Throughput Screening Assays - standards
Humans
Kinetics
Limit of Detection
Medical sciences
Molecular Probes - metabolism
Multiplex Polymerase Chain Reaction
Neoplasm Proteins
Proto-Oncogene Proteins c-abl - blood
Proto-Oncogene Proteins c-abl - genetics
Proto-Oncogene Proteins c-abl - metabolism
Reverse Transcriptase Polymerase Chain Reaction
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Summary:Reverse transcription quantitative polymerase chain reaction (RTqPCR)is currently the most sensitive tool available for the routine monitoring of disease level in patients undergoing treatment for BCRABL1 associated malignancies. Considerable effort has been invested at both the local and international levels to standardise the methodology and reporting criteria used to assess this critical metric. In an effort to accommodate the demands of increasing sample throughput and greater standardization, we adapted the current best-practice guidelines to encompass automation platforms and improved multiplex RT-qPCR technology.
ISSN:0361-8609
1096-8652
DOI:10.1002/ajh.23212