Phosphonoacetate biosynthesis: In vitro detection of a novel NADP+-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of a marine Roseobacter

A novel phosphonoacetaldehyde-oxidizing activity was detected in cell-extracts of the marine bacterium Roseovarius nubinhibens ISM grown on 2-aminoethylphosphonic acid (2-AEP; ciliatine). Extracts also contained 2-AEP transaminase and phosphonoacetate hydrolase activities. These findings indicate th...

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Bibliographic Details
Published in:Microbiology (New York) 2011-06, Vol.80 (3), p.335-340
Main Authors: Cooley, N. A., Kulakova, A. N., Villarreal-Chiu, J. F., Gilbert, J. A., McGrath, J. W., Quinn, J. P.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Experimental Articles
Life Sciences
Medical Microbiology
Microbiology
Roseobacter
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Summary:A novel phosphonoacetaldehyde-oxidizing activity was detected in cell-extracts of the marine bacterium Roseovarius nubinhibens ISM grown on 2-aminoethylphosphonic acid (2-AEP; ciliatine). Extracts also contained 2-AEP transaminase and phosphonoacetate hydrolase activities. These findings indicate the existence of a biological route from 2-AEP via phosphonoacetaldehyde for the production of phosphonoacetate, which has not previously been shown to be a natural product. The three enzymes appear to constitute a previously-unreported pathway for the mineralization of 2-AEP which is a potentially important source of phosphorus in the nutrient-stressed marine environment.
ISSN:0026-2617
1608-3237
DOI:10.1134/S0026261711030076