Single nucleotide polymorphism –799C/T in matrix metalloproteinase-8 promoter region in arterial disease

• Published in: Innate immunity (London, England) England), 2012-06, Vol.18 (3), p.511-517
• Main Authors: Pradhan-Palikhe, Pratikshya, Pussinen, Pirkko J, Vikatmaa, Pirkka, Palikhe, Anil, Kivimäki, Anne S, Lepäntalo, Mauri, Salo, Tuula, Sorsa, Timo
• Format: Article
• Language: English
• Subjects: Aged; Atherosclerosis - blood; Atherosclerosis - genetics; Atherosclerosis - immunology; Female; Finland; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Humans; Male; Matrix Metalloproteinase 8 - blood; Matrix Metalloproteinase 8 - genetics; Middle Aged; Polymorphism, Single Nucleotide; Promoter Regions, Genetic - genetics
• ISSN: 1753-4259; 1753-4267
• DOI: 10.1177/1753425911423852

Arterial disease is associated with elevated serum matrix metalloproteinase (MMP)-8 concentration. We studied the role of two promoter region single nucleotide polymorphisms (SNPs) of MMP-8 gene in the arterial disease. The population comprised patients with arterial disease (n = 124) and healthy blood donors (n = 100) as a reference group for MMP-8 SNPs (−799C/T and −381A/G) genotypes and serum concentrations. Genotype frequencies for MMP-8 −799C/T SNP in arterial disease were C/C (43.5%), C/T (32.3%) and T/T (24.2%), and in the reference group they were C/C (50.0%), C/T (40.0%) and T/T (10.0%; P = 0.012). The −799C allele frequency was lower in the patients (59.7%) than in the reference group (70.0%; P = 0.023). The −799C allele showed protective effects against the arterial disease with an odds ratio [95% confidence interval (CI)] of 0.372 (0.141–0.980, P = 0.045) after adjustment for age, gender, and serum MMP-8 and TIMP-1 concentrations. Only in the reference group and whole study population (n = 224), the −799TT genotype significantly associated with an increase in serum MMP-8 concentrations (P = 0.047, 0.025). The −799C allele appeared protective against the arterial disease. The genotype may have an effect on systemic MMP-8 levels which could not, however, be seen in the arterial disease patients probably as a result of the strong inflammation involved in the disease pathogenesis.