Acceleration of Tendon-Bone Healing of Anterior Cruciate Ligament Graft Using Autologous Ruptured Tissue

• Published in: The American journal of sports medicine 2012-06, Vol.40 (6), p.1296-1302
• Main Authors: Matsumoto, Tomoyuki, Kubo, Seiji, Sasaki, Ken, Kawakami, Yohei, Oka, Shinya, Sasaki, Hiroshi, Takayama, Koji, Tei, Katsumasa, Matsushita, Takehiko, Mifune, Yutaka, Kurosaka, Masahiro, Kuroda, Ryosuke
• Format: Article
• Language: English
• Subjects: Animals; Anterior Cruciate Ligament - diagnostic imaging; Anterior Cruciate Ligament - surgery; Anterior Cruciate Ligament Reconstruction - methods; Biological and medical sciences; Disease Models, Animal; Diseases of the osteoarticular system; Dogs; Ligaments; Medical sciences; Neovascularization, Physiologic - physiology; Osteoblasts - cytology; Osteoblasts - physiology; Osteogenesis - physiology; Skin & tissue grafts; Tendons; Tensile Strength; Tomography, X-Ray Computed; Transplants; Transplants & implants; Wound Healing
• ISSN: 0363-5465; 1552-3365
• DOI: 10.1177/0363546512439026

Background: It has been recently reported that human anterior cruciate ligament (ACL) ruptured tissue contains abundant vascular stem cells that contribute to tendon-bone healing in an immunodeficient rat model of ACL reconstruction. Hypothesis: Autologous ruptured ACL tissue has an effect on the maturation of bone-tendon integration in anterior cruciate ligament reconstruction. Study Design: Controlled laboratory study. Methods: Twenty healthy adult beagle dogs underwent bilateral ACL reconstruction using the ipsilateral flexor digitorum superficialis tendon and were divided into 2 groups: right knee (a tissue-treated group) and left knee (a control group). The tissue-treated group received autologous ruptured ACL tissue, which was obtained 2 days after resection and sutured to the tibial side of the graft. Histological, radiographic, and biomechanical assessments were performed. In addition, immunohistochemical staining was performed to assess angiogenesis and osteogenesis. Results: Histological assessment and staining for osteoblasts and endothelial cells at week 2 demonstrated early healing, inducing endochondral ossification-like integration with enhanced angiogenesis and osteogenesis in the tissue-treated group’s grafts. Computed tomography at week 4 showed a significantly smaller tibial bone tunnel in the tissue-treated group (tissue, 19.0 ± 4.4 mm2; control, 42.6 ± 4.7 mm2; P = .009, n = 5). Furthermore, biomechanical testing of force during loading to ultimate failure at week 4 demonstrated a significantly higher strength in the tissue-treated group (tissue, 66.4 ± 10.1 N; control, 30.5 ± 10.3 N; P = .009, n = 5). Conclusion: In the present study, the authors elucidated that transplantation of ACL-ruptured tissue, which was sutured to the tibial side of the graft, contributed to early tendon-bone healing in a canine model of ACL reconstruction. Clinical Relevance: Anterior cruciate ligament ruptured tissue has a therapeutic potential in promoting an appropriate environment for tendon-to-bone healing in bone tunnels of ACL reconstruction.