Transgenic chimera quail production by microinjecting lentiviral vector into the blood vessel of the early embryo

In the past, several strategies have been used to generate transgenic birds. The most successful method has proven to be injection of lentiviral vector into the subgerminal cavity of the newly laid egg. In this study, we directly injected lentiviral vector into the blood vessel of HH13–15 quail embr...

Full description

Saved in:
Bibliographic Details
Published in:Animal science journal 2012-04, Vol.83 (4), p.291-298
Main Authors: SUN, Peng, ZHANG, Zifu, WU, Guojin, YAN, Li, YUAN, Fang, ZHANG, Wenxin, GAO, Junshuang, JIN, Wenjing, LI, Zandong
Format: Article
Language:English
Subjects:
Animal sciences
Animals
Animals, Genetically Modified
blood
blood vessel microinjection
Blood vessels
Blotting, Southern
Blotting, Western
Cavities
Chimera - genetics
Chimeras
chimerism
Cytomegalovirus
eggs
Embryos
enhanced green fluorescent protein
genes
Genetic Vectors
Genomes
Gonads
Green fluorescent protein
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Immunohistochemistry
lentiviral vector
Lentivirus
Male
Microinjections
Polymerase Chain Reaction
primordial germ cell
Promoters
Quail - embryology
Quail - genetics
quails
Semen
Transgenic animals
transgenic chimera quail
Wildfowl
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the past, several strategies have been used to generate transgenic birds. The most successful method has proven to be injection of lentiviral vector into the subgerminal cavity of the newly laid egg. In this study, we directly injected lentiviral vector into the blood vessel of HH13–15 quail embryos to produce transgenic chimeras. In the manipulated, hatched birds, the green fluorescent protein (GFP) gene driven by a cytomegalovirus (CMV) promoter was extensively expressed. All tissues analyzed were GFP‐positive, and gonad cells from some of the manipulated embryos expressed GFP. The semen genome of 21.4% of mature male birds was determined to be GFP‐positive by PCR, indicating these male birds were transgenic chimeras.
ISSN:1344-3941
1740-0929
DOI:10.1111/j.1740-0929.2011.00959.x