Effects of erdosteine on cyclosporin-A-induced nephrotoxicity

• Published in: Human & experimental toxicology 2012-06, Vol.31 (6), p.565-573
• Main Authors: Tutanc, M, Arica, V, Yılmaz, N, Nacar, A, Zararsiz, I, Basarslan, F, Tutanc, OD, Nacar, E
• Format: Article
• Language: English
• Subjects: Albumin; Animals; Antioxidants; Antioxidants - pharmacology; Antioxidants - therapeutic use; Biological and medical sciences; Catalase; Catalase - metabolism; Creatinine; Cyclosporine - toxicity; Drugs; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Immunosuppressive Agents - toxicity; Kidney; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Kidney Diseases - chemically induced; Kidney Diseases - drug therapy; Kidney Diseases - metabolism; Kidney Diseases - pathology; Male; Malondialdehyde; Malondialdehyde - metabolism; Medical sciences; Nephrology; Nitric oxide; Nitric Oxide - metabolism; Nitrogen; Oxidants; Oxidative stress; Oxidative Stress - drug effects; Preventive medicine; Rats; Rats, Wistar; Rodents; Sodium chloride; Superoxide dismutase; Superoxide Dismutase - metabolism; Thioglycolates - pharmacology; Thioglycolates - therapeutic use; Thiophenes - pharmacology; Thiophenes - therapeutic use; Toxicity; Toxicology; Urea; Uric acid
• ISSN: 0960-3271; 1477-0903
• DOI: 10.1177/0960327111417907

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.