Ultrasensitive Colorimetric DNA Detection using a Combination of Rolling Circle Amplification and Nicking Endonuclease-Assisted Nanoparticle Amplification (NEANA)

A combination of rolling circle amplification and nicking endonuclease‐assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary seq...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2012-06, Vol.8 (12), p.1846-1850
Main Authors: Xu, Wei, Xie, Xiaoji, Li, Dawei, Yang, Zhaoqi, Li, Tianhu, Liu, Xiaogang
Format: Article
Language:English
Subjects:
Base Sequence
colorimetric detection
Colorimetry - methods
DNA
DNA - analysis
DNA - chemistry
Endonucleases - metabolism
Escherichia coli - metabolism
Gold - chemistry
gold nanoparticles
Metal Nanoparticles - chemistry
Microscopy, Atomic Force
Molecular Sequence Data
Nanoparticles - chemistry
nicking endonuclease
Nucleic Acid Amplification Techniques
Nucleic Acid Hybridization
Oligonucleotides - chemistry
rolling circle amplification
Sequence Homology, Nucleic Acid
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Summary:A combination of rolling circle amplification and nicking endonuclease‐assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary sequences at ultralow concentrations.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201200263