Expression of vascular endothelial growth factor-C in gastric carcinoma and the effect of its antisense gene transfection on the proliferation of human gastric cancer cell line SGC-7901

• Published in: The American journal of surgery 2012-07, Vol.204 (1), p.78-83
• Main Authors: Zhu, Peng, Ph.D, Zhang, Jianbo, M.S, Chen, Qi, M.S, Wang, Jijian, Ph.D, Wang, Yaxu, Ph.D
• Format: Article
• Language: English
• Subjects: Adult; Aged; Antisense eukaryotic expression vector; Biological and medical sciences; Blotting, Western; Cancer; Carcinoma - genetics; Carcinoma - metabolism; Carcinoma - pathology; Cell Line, Tumor; Cell Proliferation; Female; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression Regulation, Neoplastic; General aspects; Human gastric cancer cell line; Humans; Immunohistochemistry; Lymphangiogenesis; Lymphangiogenesis - genetics; Lymphatic Metastasis; Lymphatic system; Male; Medical sciences; Middle Aged; Plasmids; Polymerase Chain Reaction - methods; Proteins; RNA, Antisense - metabolism; RNA, Messenger - metabolism; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Studies; Surgery; Transfection; Tumors; Vascular endothelial growth factor; Vascular Endothelial Growth Factor C - genetics; Vascular Endothelial Growth Factor C - metabolism; Vascular endothelial growth factor-C
• ISSN: 0002-9610; 1879-1883
• DOI: 10.1016/j.amjsurg.2011.06.056

Abstract Purpose The aim of this study was to investigate the relationship between the expression of vascular endothelial growth factor-C (VEGF-C) in gastric carcinoma and tumor lymphangiogenesis and to determine the effect of antisense–VEGF-C gene transfection on proliferation. Methods Adjacent cancer tissues were collected from 72 gastric carcinoma cases and compared with 10 nongastric carcinoma tissues to detect the expression of VEGF-C and its messenger RNA (mRNA) and calculate the density of neonatal lymphatic microvessels. The in vitro–cultured gastric cancer cell line SGC-7901 was transfected with recombinant plasmid pCI-neo-anti VEGF-C. The expression in the transfected cells and the proliferation were determined. Results The positive rate of VEGF-C mRNA in the lymph node metastasis tissues was 85.7% compared with negative controls (20%, P < .05). The density of lymphatic vessels in the metastasis group was 6.65 ± 1.57 compared with the negative group (3.75 ± 1.47, P < .05). Protein and mRNA of VEGF-C were reduced in transfected cells. Proliferation was inhibited as well. Conclusions VEGF-C can increase the invasiveness of gastric cancer and promote lymphangiogenesis in adjacent tissues. Transfection with antisense VEGF-C can reduce the expression of VEGF-C and inhibit the proliferation. VEGF-C can inhibit the tumor growth and reduce its metastasis and recurrence.