Critical Evaluation of Phosphate Solubilizing Pseudomonads Isolated from a Partially Recultivated Potash Tailings Pile

Ten phosphate solubilizing pseudomonads isolated from a partially recultivated potash tailings pile in Germany were characterized and tested for their potential to assist in the ongoing recultivation process. Despite fertilization, the plants which are grown for recultivation show phosphate deficien...

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Bibliographic Details
Published in:Current microbiology 2012-08, Vol.65 (2), p.202-206
Main Authors: Koch, Sebastian, Majewski, Elke, Schmeisky, Helge, Schmidt, Friedrich R. J
Format: Article
Language:English
Subjects:
Bacteria
Biofertilizers
Biomedical and Life Sciences
Biotechnology
Calcium phosphates
Complex Mixtures
Culture Media - chemistry
Germany
Life Sciences
Microbiology
Mine tailings
Organic acids
organic acids and salts
organic production
Phosphates - metabolism
Plant Development
Plants - microbiology
Potash
Pseudomonas - growth & development
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Temperature
tricalcium phosphate
viability
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Summary:Ten phosphate solubilizing pseudomonads isolated from a partially recultivated potash tailings pile in Germany were characterized and tested for their potential to assist in the ongoing recultivation process. Despite fertilization, the plants which are grown for recultivation show phosphate deficiency symptoms, and therefore the isolates are intended to be used as biofertilizer inoculants. On agar plates incubated at five different temperatures, some of the strains showed a temperature-dependent ability to solubilize tricalcium phosphate, while others performed the same at any given temperature. In liquid medium, the isolates solubilized between 271 and 730 μg ml−1 of phosphate from tricalcium phosphate. Both the weakest (designated S10) and the strongest solubilizing strain (S06) were further tested for their viability during solubilization. In an assay over the course of 1 week, both strains released their maximum amount of phosphate after 2–4 days. At that later point of time, however, viable cells of isolate S06 were no longer detectable, whereas the weaker strain S10 could be cultured after 1 week in broth. Taking all in vitro observations into account, the usability of the isolates as biofertilizers is critically discussed regarding both the in situ conditions on the tailings pile and the lowered viability due to the excess production of organic acids.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-012-0145-0